This paper focuses on the micro-contact printing to replace photolithography. Exposure process is more expensive and time-consuming in photolithography. In this paper, we use the micro-contact printing technology and wetting gradient principle to generate the patterned thin film of photoresist. This technology not only increase applications of the micro-contact printing also reduce the time and cost of the photolithography process. This technology which have wetting gradient on PDMS stamp can be successfully patterned 200 μm thin film of photoresist. Pattern resolution also reached more than 95% , and can be successfully applied to etch applications on Indium tin oxide (ITO) substrate.