- 學位論文
金奈米粒子修飾於光柵作為波導模態共振生物感測器
Gold Nanoparticles-Modified Grating as Guided Mode Resonance Biosensor
摘要
本研究是將金奈米粒子，結合光柵式導模共振技術，研發新型光學式生物感測晶片。感測原理為：當滿足特定角度及波長之入射光，進入光柵式導模共振感測晶片，洩漏模態和光柵繞射條件達到相位匹配時，電場分佈在光柵表面,使修飾在光柵表面的金奈米粒子產生粒子電漿共振效應。即使僅有微量的生化分子與金奈米粒子鍵結，仍可量測到粒子電漿共振的變化，達到生物感測之目的。 在本研究中，成功的將球形金奈米粒子及棒形金奈米粒子修飾在光柵波導晶片上。球形金奈米粒子光柵波導晶片對環境折射率的感測靈敏度為2.21 RIU-1，sensor resolution為1.82×10-5RIU。用於檢測生化分子anti-DNP，偵測極限可達1.18×10-10 M。而棒形金奈米粒子光柵波導晶片對環境折射率感測靈敏度為26.61 RIU-1的sensor resolution為4.16×10-6 RIU。顯示金奈米粒子結合光柵波導模態共振生物感測器能夠免標定且即時檢測生化分子，並有良好的檢測結果。
並列摘要
Many diseases will release specific biomolecules before outbreak of the diseases. Particle plasmon resonance (PPR) occurs when a noble metal nanoparticle absorbs a specific wavelength of energy which is equal to the oscillation frequency of the conductive electrons. The principle of sensing here is based on the coupling of incident light into the guided-mode resonance sensing chip through a grating at a specific angle and wavelength. When the leakage mode and diffraction grating matching condition is reached, the electric field distribution on the grating surface will enhance the particle plasmon resonance effect of the gold nanoparticles. When the particle’s surrounding refractive index changes, the particle plasmon resonance band will shift, resulting in optical signal change. In this study, we successfully modified gold nanoparticles (GNP) and gold nanorods (GNR) on grating. The GNP-GMR-PPR sensor has achieved an absorbance sensitivity of 2.21 RIU-1 at an excitation wavelength of 532 nm and a sensor resolution of 1.82×10-5 RIU. The limit of detection for anti-DNP antibody is 1.18×10-10 M. The GNR-GMR-PPR sensor has achieved an absorbance sensitivity of 26.61 RIU-1 at an excitation wavelength of 885 nm and a sensor resolution 4.16×10-6 RIU.