優化金奈米粒子固定化製程並應用於自我校正型光纖式粒子電漿共振感測器

Optimization of Gold Nanoparticle Immobilization Process and Its Application to Self-Referencing Fiber-Optic Particle Plasmon Resonance Sensor

吳勁葦(Chin-Wei Wu)

指導教授 ： 周禮君

國立中正大學/理學院/化學暨生物化學系/博士(2015年)

摘要

粒子電漿共振特性可運用於各種感測技術，本研究使用了各種不同結構之架橋分子(APTES、MPTMS、MPDMS、MP-silatrane)來修飾溶於水溶液態之金奈米粒子，並建立了調整各種修飾參數(時間、濃度等)來改進修飾金奈米粒子之製程，修飾溶於水溶液態之金奈米粒子其最大吸收度和半高全寬之相對標準偏差皆小於10%。進一步將溶於水溶液態之金奈米粒子運用於光纖式粒子電漿共振感測器，其感測靈敏度約為7.13 RIU-1。 面對複雜樣品之檢測，運用自我校正型光纖式粒子電漿共振感測器來做為自我校正之用途，其含有雙槽道分別置有感測光纖和參考光纖，其中感測光纖上修飾有辨識分子生物素來對待測物生物素抗體進行檢測，分別針對非特異性吸附、折射率和顏色干擾來做校正。針對折射率和非特異性吸附干擾校正後之回收率約為103.2%，針對顏色和非特異性吸附干擾校正後之回收率約為93.9%，而將顏色、折射率和非特異性吸附等因素加在一起成為複雜樣品之檢測結果與單純樣品使用配對t檢定比較，在95%信心區間之下沒有顯著差異，就結果看出自我校正系統可降低外在環境之干擾。

關鍵字

架橋分子 ； 粒子電漿共振 ； 金奈米粒子 ； 光纖式粒子電漿共振感測器 ； 自我校正

並列摘要

The particle plasmon resonance (PPR) sensing technology has high potential to be applied in many research fields. This work investigated four different bridging molecules (APTES, MPTMS, MPDMS, and MP-silatrane) for anchoring of gold nanoparticle (AuNPaq) on glass and optimized the parameters in the AuNPaq anchoring process (immersing time, concentration of AuNP…etc). The relative standard deviation (RSD) of the peak extinction (extinctionmax) and the full width at half maximum (FWHM) were both less than 10%. With the AuNPaq anchored on a sensing fiber as the fiber optic plasmon resonance (FOPPR) sensor, the sensor sensitivity of the FOPPR sensor was about 7.13 RIU-1. In addition, a self-referencing fiber optic plasmon resonance (SR-FOPPR) sensor has been developed to compensate thermal and bulk-composition effects as well as nonspecific adsorption for biosensing in complex samples. The sensing chip of the SR-FOPPR sensor consists of an analysis fiber and a reference fiber. As an illustration, the analysis fiber is functionalized with biotin (probe) to detect anti-biotin (target). The recovery under the interference of refractive index change and nonspecific adsorption is 103.2%. The recovery under the interference of color and nonspecific adsorption is 93.9%. Furthermore, results from simple samples and complex samples (under interference of color, refractive index, nonspecific adsorption) are not significantly different at the 95% confidence interval by pair-t test. Such results show the SR-FOPPR sensor can compensate the interferences due to the effects of color, refractive index change, and nonspecific adsorption.

並列關鍵字

fiber optic plasmon resonance sensor ； bridging molecule ； particle plasmon resonance ； gold nanoparticle ； self-referencing

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