免標定雙光柵耦合式生物感測器之研究

本研究發新型的雙光柵耦合生物感測器，結構上利用耦入光柵及耦出光柵，分別將光耦入波導層中以及將光耦出波導層外，藉由兩次耦合的效應，增強晶片靈敏度並提升檢測能力。在製程方面使用射出成型來大量製造品質均一的雙光柵晶片，以達到快速生產及降低生產成本的目的；接著運用濺鍍方式製作波導層二氧化鈦，濺鍍除了能夠產生厚度均勻的薄膜外，我們也利用此製程製作雙面光柵（double -sided grating）結構，此種結構讓我們的雙光柵晶片對於一定範圍的耦合角度（12.39°至11.09°）與一定範圍的耦合位置（0.5 mm至5.0 mm）能夠有相同的靈敏度，具有較高的誤差容忍度，因而降低檢測的設備成本。　　由折射率感測實驗可得知雙光柵生物晶片的小範圍折射率（n=1.3355至n=1.343）蔗糖溶液實驗靈敏度平均為-46.42±2.23 RIU^-1左右，解析度則是-3.13E-6±1.11E-6 RIU (n=6)；而最佳靈敏度為-48.55 RIU^-1，最佳解析度為-1.82E-6 RIU。生化免疫反應(DNP與anti-DNP的鍵結)的檢測可以測得平均最小濃度(LOD)為3.09E-8 ±4.34E-9 g/ml (n=3)，最佳LOD為2.73E-8 g/ml。

關鍵字

免標定生物感測器；雙光柵波導耦合器；雙面光柵；折射率檢測；免疫分析

並列摘要

This study develops a novel label-free biosensor based on dual grating waveguide couplers, which have input grating and output grating to allow the laser light source to enter the waveguide and leave the waveguide, respectively. The dual gratings can enhance the biosensor’s sensitivity and improve the detection limit. In terms of fabrication processes, injection molding and sputtering are used. Injection molding of cyclic olefin copolymer (COC) biosensors provides uniform quality and mass production capability with the following features: rapid production and decreasing the fabrication cost. Sputtering was used tofabricateTiO2 waveguide layer and double-sided grating waveguide. By using double-sided grating waveguide, this biosensor provides high position error tolerance in the incident angle and coupling: incident angle from 12.39°to 11.09°and coupling position from 0.5 mm to 5.0 mm have the same sensitivities. Furthermore, this feature is able to reduce the cost of equipment. This dual grating waveguide coupler biosensor demonstrates the capability in refractive-index detection through sucrose solution, and immunoassay of dinitrophenyl (DNP)/anti-DNP. Using small index change of sucrose solution (n=1.3355~ n=1.343), the measured average sensitivity and resolution are -46.42±2.23 RIU^-1 and -3.13E-6±1.11E-6 RIU(n=6), respectively. The best sensitivity is -48.55 RIU^-1 and the best resolution is -1.82E-6 RIU. The measured average limit of detection (LOD)is 3.09E-8 ±4.34E-9 g/ml (n=3) and the best LOD is 2.73E-8 g/ml through DNP/anti- DNP interaction.

並列關鍵字

Label-Free Biosensor ； Dual grating waveguide couplers ； Double-sided grating ； Refractive index detection ； Immunoassay

參考文獻

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免標定雙光柵耦合式生物感測器之研究

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