不同栽培方法對牛樟芝菌絲生長之影響

Effects of Different Culture Media and Conditions on the Mycelial Growth of Antrodia camphorata T. T. Chang & W. N. Chou

施玉蘭(Yu-Lan Shih)

指導教授 ： 李嘉偉 ； 羅聖賢

國立屏東科技大學/農學院/熱帶農業暨國際合作系所/碩士(2009年)

https://doi.org/10.6346/NPUST.2009.00062

摘要

本研究以台灣特有真菌中的牛樟芝（Antrodia camphorata T. T. Chang & W. N. Chou）為題材，研究進行不同栽培方法對牛樟芝菌絲生長之影響，探討促使菌絲體成長的最佳條件與程序，建立完整之栽培模式，作為日後栽培牛樟芝提供商業生產之依據。本實驗聚焦於在不同培養基和溫度對牛樟芝菌絲生長的影響。牛樟芝菌絲在純的PDA（potato dextrose agar）上於26與28 oC者生長速率相等且較30 oC者快。在純PDA及分別添加麥芽萃取物（malt extract）、酵母萃取物（yeast extract）及牛樟木屑浸出液（camphorata broth）在第21天菌絲生長平均直徑依遞減排列為添加malt extract (3.45 cm)，純PDA (2.95 cm)，添加yeast extract（0.1 cm）。三者間有顯著差異（p < 0.05）；添加camphorata broth（3.20 cm） 與PDA及添加malt extract無顯著差異（p > 0.05），但與添加yeast extract 有顯著差異（p < 0.05），PDA添加malt extract及牛樟木屑浸出液可以增快 A. camphorata 菌絲生長速率。添加麥芽萃取物的量40 g/L、30 g/L、20 g/L對菌絲的生長無顯著差異（p > 0.05），但菌絲的生長隨添加牛樟木屑浸出液的量增多而增大。麥粒對菌絲的生長不論添加牛樟木屑浸出液與否均為不良的基質，將糙米與麥粒菌絲生長平均直徑做比較第40天為糙米100 mL（136 g）加60 mL牛樟木屑浸出液（5.56 cm）> 麥粒100 mL（90 g）加60 mL牛樟木屑浸出液（1.75 cm），兩者間有顯著差異（p < 0.05），糙米是較好的基質。牛樟芝菌絲在同樣是糙米加冇樟木屑的基質中加野生牛樟木浸出液中比加人工栽培牛樟木浸出液生長效果明顯優異。

關鍵字

牛樟芝 ； PDA ； 牛樟木屑浸出液 ； 麥芽萃取物 ； 酵母萃取物

並列摘要

This study investigated effects of different culture media and conditions on the growth of mycelia of a specific fungus in Taiwan, Antrodia camphorata T. T. Chang & W. N. Chou. The main purpose of this study is to find out the best conditions and procedures for the growth of A. camphorata mycelia , then setup a complete model for culturing A. camphorata and even commercial production. This study focused on the effects of different culture media and temperature conditions on the growth of Atrodia camphorate. The mycelia of A. camphorata had same growing rate in PDA (potato dextrose agar) at 26 and 28 oC and both are better than at 30 oC. The descending order of mean diameters of mycelia on the 21st day after inoculating A. camphorata on PDA, PDA + malt ectract, PDA + yeast extract, and PDA + camphorata broth were PDA + malt extract (3.45 cm) > PDA (2.95 cm) > PDA + yeast ectract (0.1 cm) and there were significant different among the three data sets (p < 0.05); however, PDA + camphoratebroth (3.20 cm) was not significant different from PDA and PDA+malt extract (p > 0.05). PDA with malt extract and camphorate broth can definitely improve the growing rate of A. camphorata mycelia. When the dosages of malt extract that added to PDA varies from 20 g/L to 30 g/L to 40 g/L, the mycelia growing rates are not significantly different from each other (p > 0.05); however, the the higher is the dosage of camphorata broth, the higher is the growing rate of the mycelia . Wheat grain was not a good substrate for A. camphorata mycelia growing no matter whether the grain were added the camphorata broth or not. The 40th days records of average diameters of the mycelia growing on 100 mL (136 g) brown rice added by 60 mL camphorata broth (5.52 cm) was higher than 100 mL (90 g) wheat grain added by 60 mL camphorata broth (1.75 cm), and there was significant difference between them. Brown rice is a good substrate for the camphorata mycelia growth. Wild C. kaneherai wood is better than cultured C. kaneherai wood for A. camphorata mycelia growing.

並列關鍵字

Antrodia camphorata T. T. Chang & W. N. Chou ； PDA ； camphorata broth ； malt extract ； yeast extract

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