蚌蘭萃取物的藥理活性研究與其沒食子酸含量分析

The Study on the pharmacological effect and the analysis of gallic acid in Rhoeo discolor extracts

童熯民(Ham-Ming Tong)

指導教授 ： 蔡清恩

國立屏東科技大學/農學院/獸醫學系所/碩士(2009年)

https://doi.org/10.6346/NPUST.2009.00180

摘要

蚌蘭（Rhoeo discolor）是民間常用的草藥，原產於墨西哥多年生草本植物，栽培於庭院或花圃做為觀賞或藥用。據文獻(M. Gonza, 2003)指出蚌蘭葉片之乙醇粗萃取物具有抗氧化(antioxidant)、抗突變(antimutagenic)以及抑制肝癌化的作用。本研究首先選用18株獸醫臨床上常見之微生物，以紙錠擴散法與最小抑制濃度(MIC)試驗法測試蚌蘭水萃取物對於抑制微生物生長之活性實驗。在抗纖維化試驗方面，大鼠以化學藥物誘導肝纖維化，使用四氯化碳(50 %, l.0 mL/kg)一周三次，以誘導大鼠肝臟的纖維化，蚌蘭水萃取物(100 mg/kg)每日一次，藥理活性評估指標包括臟器重量/體重比値及組織病理學變化之差異性。蚌蘭中沒食子酸含量分析為使用5N的鹽酸溶液萃取，利用高效能液相層析儀以及連接串聯式質譜儀分析。結果顯示水層的蚌蘭甲醇萃取物具有抑制微生物生長的活性，而正丁醇層與乙酸乙酯層則無抑制活性；其中以Streptococcus agalactiae 與 Streptococcus uberi具有較大的抑制圈(30 mm)。MIC試驗結果為0.3125 ~ 10 mg/mL，其中以Staphylococcus aureus 與Micrococcus luteus具有較小的抑制濃度。在抗纖維化試驗結果，肝/體重與腎/體重比値方面，蚌蘭組(3.2 ± 0.1；0.69 ± 0.06)較四氯化碳組(3.5 ± 0.16；0.76 ± 0.05)低( p < 0.05)，在組織病理學方面，蚌蘭水萃取物能降低由CCl4誘發肝纖維化之膠原蛋白堆積(collagen accumulation)(p < 0.01)的現象。蚌蘭含沒食子酸分析方面，可得每克蚌蘭中有1.007 mg沒食子酸的含量。

關鍵字

蚌蘭 ； 抗微生物活性 ； 抗纖維化 ； 沒食子酸

並列摘要

Rhoeo discolor is a legendary plant used for treatment of superficial mycoses infection in Mexican traditional medicine. The Rhoeo discolor crude extract to be provided with antioxidant, antimutagenic. The antimicrobial activities of the Rhoeo discolor extracts, which against 18 clinical bacterial species in veterinary medicine were tested by using disc diffusion assay and minimal inhibition concentration (MIC) test. The liver fibrosis in adult Sprague-Dawley rats was induced by carbon tetrachloride (50 %, l.0 mL/kg, by gavage) thrice a week for 6 weeks. The gallic acid of Rhoeo discolor was extracted with 5N HCl and detected by HPLC and LC/MS/MS method. The results showed that water soluble of Rhoeo discolor with MeOH extract had antibacterial activity against all strains of bacterias. The Streptococcus agalactic and Streptococcus uberi have the biggest inhibitory zone (30 mm). whereas the n-BuOH and EtOAc soluble are negative. The MIC value was shown 0.3125 ~ 10 mg/mL. The Staphylococcus aureus has the smallest inhibition concentration. The results of histopathogical examination showed Rhoeo discolor extracts (100 mg/kg) can significantly decreased the ration of liver to body weight (p < 0.05), and the degree of hepatocytic degeneration (p < 0.01) and liver fibrosis, (p < 0.05). The concentration of gallic acid detected by HPLC method in Rhoeo discolor was 1.007 mg/g.

並列關鍵字

Rhoeo discolor ； antimicrobial activities ； liver fibrosis ； gallic acid

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