樟芝(Antrodia cinnamomea)固態發酵培養產物之成分分析與抗氧化活性之研究

Study on the Component Analysis and Antioxidant Activity from Product of Solid Fermentation Culture in Antrodia cinnamomea

王柏森(Bo-Sen Wang)

指導教授 ： 賴宏亮 博士 ； 張誌益 博士

國立屏東科技大學/農學院/生物科技研究所/碩士(2008年)

https://doi.org/10.6346/NPUST.2008.00117

摘要

樟芝(Antrodia cinnamomea)為台灣特有的大型真菌類，價格昂貴，生長於牛樟樹內壁且速度緩慢，具有解毒、抗癌等功效。本研究之主要目的為進行樟芝菌種之分離，利用固態發酵培養基進行樟芝培養產物的試驗，並以此探討穀物、採收時間及不同萃取條件，建立固體發酵培養條件之生產體系，著眼於樟芝菌絲體成分之單離純化，經管柱層析分離到之結晶物為4,7-dimethoxy-5-methyl-1,3-benzodioxole (DMB)，以此成分為指標進行各種固態發酵產物之HPLC 成分含量定量分析。並以 Folin-ciocalteu 方法測定總酚含量，檢測 DPPH 清除能力作為其抗氧化性質的評估方法。結果顯示，樟芝菌絲體於不同穀類的培養基中生長，以培養於米並添加 ME 培養基中可以得到較多的菌絲產量。DPPH 的清除力則是以培養於小麥培養基的樟芝菌絲體 methanol 萃取物為較佳，其 SC50 為240 μg/mL。總酚測定方面亦顯示出培養於小麥並添加 ME 的培養基樟芝菌絲體 methanol 萃取物，其含有較高的總酚含量，達 57.52 mg gallic acid eq./g D.W.。由研究的結果得知，本 HPLC 分析方法具有良好的分離效果，可作為樟芝菌絲體 DMB 之定量分析條件，以提供其品質評價上的依據。其他研究結果亦顯示出樟芝穀物培養中所殘留下來的培養基部分也具有相當的抗氧化能力，具有開發的潛力。

關鍵字

樟芝(Antrodia cinnamomea) ； 總酚 ； DPPH 清除力 ； DMB ； 牛樟(Cinnamomum kanehirai)

並列摘要

Antrodia cinnamomea is a unique macrofungi in Taiwan. It’s very expensive because A. cinnamomea grows on the inner wall of Cinnamomum kanehirai and the growth rate is very slow. It has the desirable detoxification and anti-cancer properties. The purpose of this research is to isolate the strain of A. cinnamomea, purify and isolate component of mycelia in A. cinnamomea and use solid fermentation media to proceed the test of culturing product from A. cinnamomea. We can discuss different grains, harvest times, extraction conditions and establish the productive system of solid fermentation culture. We isolated one kind of crystal which was 4,7-dimethoxy-5- methyl-1,3-benzodioxole (DMB) through column chromatography. We regarded this component as the standard in order to assay and quantify the content of various solid fermentation products by HPLC. We used Folin-ciocalteu method to determine the content of total phenols. We detected the DPPH scavenging ability, so as to evaluate its antioxidant property. Besides, the results revealed that the higher yield of mycelia was from the medium which contained rice and ME. The methanol extract of A. cinnamomea mycelia from wheat medium had higher DPPH scavenging ability and the value of SC50 was 240 μg/mL. The results revealed the methanol extract of A. cinnamomea mycelia from wheat (ME) medium also had higher content of total phenols, reached 57.52 mg gallic acid eq./g D.W.. We can understand by the results, this HPLC method has a fine separating effect so that it can be regard as the condition of quantitative assay of DMB from A. cinnamomea mycelia and provide a basis of its quality evaluation. Other studies also revealed the media residue contained antioxidant ability so that the media residue can be provided with the exploration potential.

並列關鍵字

Antrodia cinnamomea ； total phenols ； DPPH scavenging ability ； 4,7-dimethoxy-5-methyl-1,3-benzodioxole (DMB) ； Cinnamomum kanehirai

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