犬隻細胞激素表現與慢性瓣膜性心臟病之相關性

Relationship between Cytokines Expression and Chronic Valvular Heart Disease of Dogs

陳慧珍(Hui-Jane Chen)

指導教授 ： 江強華

國立屏東科技大學/農學院/獸醫學系所/碩士(2008年)

https://doi.org/10.6346/NPUST.2008.00194

摘要

犬隻慢性瓣膜性心臟病是狗鬱血性心衰竭最常見的原因；此疾病亦可稱為慢性退行性瓣膜疾病。犬隻慢性瓣膜性心臟病常見於小型至中型犬。本疾病的臨床症狀有咳嗽、夜間呼吸困難、睡眠習慣改變及運動不耐。目前真正的病因還未被完全證實。由文獻可知，當心肌組織受損時可促使心肌黏附因子的表現，並誘發細胞激素的釋放，進而造成白血球及內皮細胞的活化。本實驗目的以探討犬隻慢性瓣膜性心臟病，在不同病程階段與細胞激素表現之相關性。此次實驗結果顯示，第一級慢性瓣膜性心臟病犬血漿TNF-α平均濃度為361.6 ± 78.3 pg/mL、第二級平均濃度為143.1 ± 10.3 pg/mL、第三級平均濃度為127.8 ± 17.4 pg/mL；對照組之TNF-α平均濃度為21.3 ± 3.3 pg/mL ( p<0.05 )。而在第一級慢性瓣膜性心臟病犬血漿IL-6平均濃度為116.9 ± 37.4 pg/mL、第二級平均濃度為62.1 ± 8.4 pg/mL、第三級平均濃度為88.7 ± 17.1 pg/mL；對照組之IL-6平均濃度為87.2 ± 22.9 pg/mL ( p>0.05 )。由以上結果顯示在犬隻慢性瓣膜性心臟病的分級上TNF-α比IL-6更具敏感性，且TNF-α在慢性瓣膜性心臟病之早期階段較具敏感性。

關鍵字

慢性瓣膜性心臟病 ； 細胞激素 ； TNF-α ； Interleukin-6 ； 犬

並列摘要

Chronic valve disease ( CVD ) has also been termed chronic degenerative valvular disease. The Chronic valve disease is most common in small to medium sized breeds of dogs. This disease is characterized clinically by the cough, nocturnal dyspnea, altered sleep habits, abdominal distention or exercise intolerance. But the pathogenesis is unknown. Recent literatures indicate that the cardiomyocyte injury requires the expression of myocyte intercellular adhesion molecule adhesion, and lead to release of cytokines is crucial in inducing leukocytes and endothelial cells activation. This present study is conducted to compare the relationship between cytokines expression and various phases in CVD of dogs. The results showed that the plasma average levels of TNF-α in group I were 361.6 ± 78.3 pg/mL, 143.1 ± 10.3 pg/mL in group II, and 127.8 ± 17.4 pg/mL in group III. The plasma levels of TNF-α in control group were 21.3 ± 3.3 pg/mL ( p<0.05 ). With IL-6 assays, the plasma levels of IL-6 in group I were 116.9 ± 37.4 pg/mL, 62.1 ± 8.4 pg/mL in group II, and 88.7 ± 17.1 pg/mL in group III. The plasma levels of IL-6 were 87.2 ± 22.9 pg/mL in the control group ( p>0.05 ). Therefore, these results implicate that TNF-α were more sensitivity than IL-6 in classify the stage of CVD of dog, and the TNF-α was more sensitivity in the early phase.

並列關鍵字

Chronic valve disease ； cytokines ； TNF-α ； Interleukin-6 ； dog

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