膠原胺基葡聚醣移植物在角膜傷口修復之效益

The Healing Effect of the Collagen-glycosaminoglycan Copolymer on the Corneal Wound

潘建壹(Chien-Yi Pan)

指導教授 ： 杜杰憲

國立屏東科技大學/農學院/獸醫學系所/碩士(2010年)

https://doi.org/10.6346/NPUST.2010.00076

摘要

在臨床上已證實角膜基質受損後是無法再生，而受傷角膜再生的厚度非常重要，它能阻止持續性角膜上皮缺陷或其他潛在疾病導致角膜穿孔，甚至角膜瘢痕形成或血管新生導致角膜混濁，角膜基質組織的修復是挽救眼球穿孔的主要因素。膠原胺基葡聚醣移植物，具有立體結構可被生物體吸收分解，並提供一個新的生物環境讓細胞遷移、擴散和分化，用於改善或修復無法癒合的創傷或較大的缺損傷口。先前之研究，膠原胺基葡聚醣移植物已被運用在神經、局部皮膚的真皮層再生及結膜傷口之治療；本研究，將探討膠原胺基葡聚醣移植片移植在成兔角膜傷口之癒合過程。本實驗用7.5 mm 圓鋸刀在成兔角膜上製造充份厚度之傷口，將膠原胺基葡聚醣移植物植入，以裂隙燈、組織病理學、免疫化學染色（α-SMA染色法)及測量傷口癒合厚度來評估角膜上皮和基質之修復情形；實驗結果顯示，有放置膠原胺基葡聚醣移植物之角膜傷口，其修復之角膜癒合厚度較厚，修復情形較佳。由實驗可證實組織工程（膠原胺基葡聚醣移植物)能成功的修復受損角膜基質組織的厚度，而其導致角膜不清澈和血管新生現象是可接受的缺點。

關鍵字

角膜基質 ； 角膜潰瘍 ； 膠原胺基葡聚醣 ； 兔

並列摘要

Background: The non- regeneration of injured corneal stroma is clinically confirmed. Corneal perforation directly related to the thickness of corneal stroma under the condition of corneal epithelium defect. Even the corneal scar formation and vessels ingrow cause the opacity of the cornea, the befit of preventing corneal perforation is still important. The 3-dimensional collagen-glycosaminoglycan scaffold can offer a new environment for cell migration, proliferation and differentiation which improve the large wound healing. Based on several previous studies, tissue engineering were successfully applied on the regeneration of dermis, conjuncitva and nerve.
Methods: In this study, the regeneration of surgical induced rabbit corneal defect implanted with collagen-glycosaminoglycan scaffold was proceeded. The corneal partial thickness wound was created by 7.5mm trephine. The wound was refilled by collagen-glycosaminoglycan scaffold. Clinical data included the external photographs of epithelial migration and
corneal thickness. The histological data was collected by Masson’s trichrome and alpha smooth muscle stains.
Results: The corneal stroma thickness was significantly different between the two groups. The thickness of injured cornea of collagen-glycosaminoglycan scaffold was thicker than the control group.
Discussion: The tissue engineering could successfully regain the damaged corneal stroma thickness with the acceptable corneal hazeness and neovasulariztion.

並列關鍵字

Corneal stroma ； Corneal ulcers ； Collagen–glycosaminoglycan ； rabbit

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