- 學位論文
發霉玉米、DDGS、精液pH及背脂厚度對公豬精液品質及性行為之影響
Effects of Contaminated Corn, DDGS, Semen pH, and
摘要
本研究之目的在探討發霉玉米、DDGS、精液pH值和背脂厚度對公豬精液品質和性行為之影響。此研究分為兩個實驗。這兩個實驗使用8頭約10月齡的雜交公豬（杜洛克×藍瑞斯×約克夏）。實驗一的第一階段為培訓公豬並能成功採集精液，而第二階段是餵飼DDGS和控制组飼料，從而測試它們的精液品質和性行為。在DDGS飼料中的黴菌毒素為50-120 ppm的黃麴毒素、222 ppm的嘔吐黴素和10-200 ppm的伏馬菌素。實驗一之試驗設計採完全逢機區集法。公豬隨機分配到控制组和DDGS處理组。每頭公豬為一重複，每處理共4重複，實驗為期10週。實驗二是繼續進行實驗一，但使用發霉玉米為處理组，實驗為期6週。該毒素的污染濃度為 1.7 ppm的玉米黃麴毒素，1550 ppm的嘔吐黴素，800 ppm的伏馬菌素，1.6 ppm的赭曲黴毒素。使用同樣的8頭公豬，但有三處理組：對照組，25%和50%發霉玉米。這8頭公豬隨機分配至三處理組：2頭公豬至控制組，6頭分別至25%和50%發霉玉米處理組。實驗一和二每週進行精液收集和評估、行為觀察、秤重、測量背脂厚度。收集兩個實驗中所有的數據進行統計分析。處理之影響、精液pH值、背脂厚度以ANOVA分析。處理組之間的精液品質和行為的平均值差異以鄧肯氏新多次變域法比較。實驗一的結果顯示DDGS處理组對於總活力，存活率以及精子活力的平均值有顯著差異（P<0.05，P<0.01，P<0.05）。其它的精液品質和性行為特徵並沒有顯著影響（P>0.05）。實驗二的結果顯示處理组對於總活力、存活率、精子活力有顯著的影響（分別為P<0.001，P<0.01，P<0.001），但只有餵飼25%受污染玉米處 理組極度受影響。不成功的駕乘有顯著的影響（P<0.05），和餵飼50%發霉玉米處理組的公豬與其它處理組相比，不成功的駕乘行為受影響最嚴重。大體而言，兩個實驗之處理組對公豬精液品質和性行為没有重大的影響。然而，有趣的是精液pH值和背脂厚度對於公豬的精液品質和性行為特徵有較大的影響。公豬精液的pH值在6至7.4範圍内對精液品質和性行為有較好的结果。背脂（P2）厚度也有影響公豬精液品質和性行為的趨势。公豬的背脂厚度> 11毫米對精液品質和性行為有顯著的影響。
並列摘要
The objective of this research study was to investigate the effects of contaminated corn, DDGS, semen pH, and backfat on boar semen quality and sexual behavior. Two sets of experiments were conducted for the project. For both sets of experiments, 8 hybrid boars (Landrace × Yorkshire × Duroc), aged about 10 months were used. The first phase of experiment 1 involved training of the boars for successful semen collection while the second phase involved feeding of DDGS and control diets; thereby test for their semen quality and sexual behavior performances. The contamination levels of mycotoxin in the DDGS diet were 50-120 ppm Aflatoxin, 222 ppm Vomitoxin, and 10-200 ppm Fumonisin. The treatment and design structures used in experiment 1 were one-way classification and Completely Randomized method respectively. The boars were randomly assigned to the control and DDGS treatments. Each boar was considered a replicate, and there were four replications per treatment. Period length was 10 weeks. Experiment II was a continuation of experiment 1 but contaminated corn (CC) was used as a treatment diet for a period of 6 weeks. The mycotoxin concentrations in the contaminated corn were 1.7 ppm Aflatoxin, 1,550 ppm Vomitoxin, 800 ppm Fumonisin, and 1.6 ppm Ochratoxin. The same eight boars were used, but with three (3) treatment groups: control, 25%, and 50% CC - one factor with 3 levels. The eight boars were randomly assigned to the three sets of treatments: 2 boars on the control, 3 on the 25%, and 3 on the 50% CC respectively. Semen collections and assessments, behavior observations, weighing, and back fat thickness measurements were carried out on weekly basis for both experiments one and two. All data from the two experiments were gathered and statistically analyzed. The effect of treatments, semen pH, and back fat thickness were evaluated by either T-test or analysis of variance. The differences among the means of the semen quality and sexual behavior variables were compared using the Duncan’s Multiple Range Test. Results in experiment one for “treatment” showed significant differences in mean values for gross motility, survival rate, and the individual motility of sperms (<0.05, <0.01, <0.05 respectively) but were in favor of the DDGS treatment group. Other semen quality and sexual behavior characteristics were not significantly affected (>0.05). The results in experiment II for “treatment” showed that gross motility, survival rate, and individual motility were significantly affected by the treatments (<0.001, <0.01, and <0.001 respectively) but only the group that was fed the 25% CC was most affected. False mounting was significantly affected (<0.05), and boars fed the 50% CC were most affected in false mounting behavior than those fed the 25% CC and the control diets. Generally, the treatments for both experiments one and two showed no major influence on the semen quality and sexual behavior of the boars. However, interestingly the semen pH and backfat thickness had greater influence over the semen quality and sexual behavior characteristics of the boars. Boars that had the semen pH ranging from 6 to 7.4 obtained better results in relation to semen quality and sexual behavior performances. Backfat (P2) thickness also tended to influence the boar semen quality and sexual behavior performances. Boars that had backfat thickness of >11 mm score significant results for semen quality and sexual behavior performances.