壬基苯酚與辛基苯酚於豬場地下水之污染狀況及其對豬隻免 疫細胞功能之影響

The contamination status of nonylphenol and octylphenol in groundwater in pig herds and their impacts on pig immune cell function

邱泰舜(Tai-Shun Chiou)

指導教授 ： 邱明堂

國立屏東科技大學/農學院/獸醫學系所/碩士(2011年)

https://doi.org/10.6346/NPUST.2011.00119

摘要

壬基苯酚 (nonylphenol; NP)及辛基苯酚 (octylphenol; OP)，是目前台灣常見的環境荷爾蒙 (environmental hormones; EHs)，許多河川中都可檢測出此類物質，高屏溪附近支流更可測得高濃度之NP及OP。為了解豬隻飲用水中是否亦有NP及OP之污染，及其對豬隻肺泡巨噬細胞(alveolar macrophage; AM)及周邊血液單核球(peripheral blood mononuclear cell; PBMC)功能之影響，實驗分成三部份進行。一、針對污染河川附近豬場採集豬隻飲用地下水，檢測NP及OP含量。二、採集污染豬場內之豬隻血液，檢驗血中NP及OP含量。三、活體外檢測NP及OP對豬隻AM及PBMC之存活率、吞噬率、增殖率、細胞激素分泌量及細胞凋亡之影響。結果顯示部份豬場中豬隻飲用之地下水含有不等濃度的NP及OP，同時豬隻血液中也可測得NP及OP。而當AM及PBMC暴露在OP 10 ppm 6小時的環境後，存活率顯著下降 (p＜0.05)。AM暴露在NP及OP 10、1及0.1 ppm ，24及48小時後，吞噬率呈現顯著抑制現象 (p＜0.05)。PBMC增殖率在10 ppm 之NP及OP 感作6小時後
亦明顯受到抑制 （p＜0.05)；此外，NP及OP亦可引發AM及PBMC之細胞凋亡現象。以10 ppm之NP刺激6小時後，AM分泌腫瘤壞死因子-α (TNF-α) 能力顯著提升 (p＜0.05)，若以OP或者1及0.1 ppm 之NP 刺激6小時後，其分泌TNF-α能力則顯著受抑制 (p＜0.05)，然而不論以NP或者OP刺激PBMC後，其釋放干擾素-γ (IFN-γ) 能力則無顯著差異。上述結果顯示，部份豬場地下水已受NP及OP污染，且NP及OP會於豬體內蓄積，並可導致AM及PBMC功能異常。

關鍵字

環境荷爾蒙 ； 壬基苯酚 ； 辛基苯酚 ； 肺泡巨噬細胞 ； 周邊血液單核球

並列摘要

Nonylphenol (NP) and octylphenol (OP), two well-known environmental hormones (EHs), have been detected in Taiwan. Current studies revealed the Kao-Ping River is one of the polluted areas. The goals of this project were to determine the pollution level of NP and OP in groundwater and pigs collected from pig herds beside the Kao-Ping River, and evaluate the effect of these pollutants on immune functions of porcine alveolar macrophages (AM) and peripheral blood mononuclear cells (PBMC). The experiment was divided into three parts. Firstly, to determine the NP and OP concentration in groundwater that was collected from pig herds. Second, to detect the NP and OP concentrations in blood samples obtained from pigs raised on these herds. Third, to evaluate the impact of NP and OP on the AM and PBMC immune functions in vitro. The results shown, NP and OP were detected from groundwater collected from varies pig herds. These two pollutants also detected from the blood samples of pig. Cell viability was significant
decreased when AM and PBMC exposed under 10 ppm OP after 6 hours (p＜0.05). Down regulation of AM phagocytosis function was detected under treated with NP or OP with 10, 1, and 0.1 ppm after 24 and 48 hours (p＜0.05). PBMC proliferation ability was suppressed after exposed into 10 ppm NP or OP for 6 hours (p＜0.05). In addition, NP and OP could induce AM and PBMC apoptosis rather than necrosis. On the other hand, AM secretion tumor necrosis factor-α (TNF-α) ability was significantly enhance after exposed NP 10 ppm 6 hours (p＜0.05), whatever, AM stimulation with OP or NP 1 and 0.1 ppm after 6 hours, secretion TNF-α ability was significantly suppressed (p＜0.05). In contrast, neither NP nor OP co-cultured with PBMC, the interferon-γ secretion was unaffected. These results indicate that NP and OP could detect from groundwater and accumulated in the pig blood and impaired AM and PBMC functions.

並列關鍵字

environmental hormone ； nonylphenol ； octylphenol ； alveolar macrophage ； peripheral blood mononuclear cell

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