金黃色葡萄球菌生長因子參數探討

Discussion on parameters of Staphylococcus aureus Growth

黃世仰(Shih-Yang Huang)

指導教授 ： 蔡循恒

國立屏東科技大學/工學院/生物機電工程系所/碩士(2011年)

https://doi.org/10.6346/NPUST.2011.00173

摘要

本研究將藉由多種變數之統計分析方法，探討對金黃色葡萄球菌生長之主要影響因子，將實驗分為兩部分共四組進行試驗，第一部分為環境因子對照組，不進行電刺激步驟，藉以觀測各種環境因子包含溫度、濕度、酸鹼度與培養基濃度等四項因子對菌種生長之影響並探討其最佳生長參數，再以此最佳參數進行後續實驗，而第二部分電刺激因子組視不同電流型態予以區分為直流、直流脈衝與交流電流三組。實驗結果顯示，影響金黃色葡萄球菌生長之環境參數中溫度之影響最大。而在電刺激因子組實驗中發現，電場強度因子對金黃色葡萄球菌生長之影響最大，電場強度值越高，其抑菌能力越好，且對金黃色葡萄球菌施加交流電刺激時，頻率因子之影響程度較施加直流脈衝電刺激時要高。
根據本研究進行之電刺激對金黃色葡萄球菌生長影響之試驗結果，施加電刺激確實能有效降低金黃色葡萄球菌之生長菌數。

關鍵字

金黃色葡萄球菌 ； 環境因子 ； 電刺激因子

並列摘要

This study will examine the growth of Staphylococcus aureus principal impact factors with a variety of variables statistical analysis. The experiment was divided into four groups of two tests. The first part of the environmental factors for the control group, Observed a variety of environmental factors including temperature, humidity, pH and medium concentration the growth of four factors on the impact of bacteria without electrical stimulation procedure. The second part of the electrical stimulation of the factor group depending on the type were divided into DC current, DC pulse and alternating current three groups. The results show that affect the growth of Staphylococcus aureus in the temperature of the environmental parameters most affected. In electrical stimulation experiments in the factor group found that the electric field intensity factor for the growth of Staphylococcus aureus greatest impact. The higher the field strength value, the better its antimicrobial activity. Applied AC stimulus in Staphylococcus aureus, the frequency factor is higher than the effect of electrical stimulation applied DC pulse. According to the studies of electrical stimulation on the growth of Staphylococcus aureus impact test results, applied electrical stimulation can indeed reduce the growth of Staphylococcus aureus bacteria.

並列關鍵字

Staphylococcus aureus ； environmental factors ； stimulation factors

參考文獻

26. 陳蓁湉，2009，電刺激殺菌對金黃色葡萄球菌之應用，碩士論文，國立屏東科技大學，生物機電工程研究所，屏東。

1. Szuminsky N.J., Albers A.C., Unger P., Eddy J.G., 1994, ” Effect of narrow,pulsed high voltages on bacterial viability” Phys. Ther. . Vol. 74, pp. 660-667.

2. Li, Y., C. L. Griffis, M. F. Slavik, P. V. Engler and R. E. Wolfe. 1991. ”Effects of current, frequency and duty cycle on killing Salmonella insaline water using electric signals.” Amer. Soc. Agr. Engineers. Vol. 7, pp. 705-710.

3. Bawcom, D. W., L. D. Thompson, M. F. Miller and C. B. Ramsey., 1995, “Reduction of microorganisms on beef surfaces utilizing electricity.” J.Food Prot. Vol. 58, pp. 35-38.

4. Lin, C.K., Kennick, W.H., Sandine, W.E. and Koohmaraie, M., 1984, “Effect of electrical stimulation on meat microflora: observations on agar media, in suspensions and on beef carcases.” J. Food Prot. Vol. 47, pp. 279-283