犬異位性皮膚炎診斷與減敏療法之研究

Studies on Diagnosis and Allergen-Specific Immunotherapy of Canine Atopy

朱明泉(Ming-Chuan Chu)

指導教授 ： 張清棟

國立屏東科技大學/農學院/獸醫學系所/碩士(2011年)

https://doi.org/10.6346/NPUST.2011.00245

摘要

頑固型的犬異位性皮膚炎疾病造成臨床獸醫師診療上極大的困擾，所以我們要找出較容易診斷及有效安全治療的方法。本研究自2007年10月至2008年6月間，於收集動物醫院臨床病犬參與本試驗，並隨機選取24隻病犬分並成3組：第一組為陰性對照組；第二組為陽性未治療組；第三組為陽性注射減敏針治療組。實驗開始時，每隻病犬皆檢驗血清中所含IgG、IgE總量及特異過敏原IgE含量，以及一般全身血液生化及血球分析和寄生蟲檢驗等健康評估，同時進行皮膚切片檢查，顯微鏡下觀察紀錄皮膚組織細胞特徵，治療組給予施行減敏針注射計劃，爾後每個月再抽血檢驗血清中所含IgG、IgE總量，連續九個月後再監測特異過敏原IgE含量，並重複地進行皮膚切片檢查其組織細胞特徵性之變化，蒐集以上各項數據後以生物統計學方法計算分析其相關性，同時再檢測一般全身血液生化及血球分析和寄生蟲檢驗等健康評估作為此治療實驗安全性評估之依據。實驗結果在血清學IgG及IgE總量之測定統計分析，顯示其各組比較均無顯著差異 (P值>0.0167) 。在組織病理學觀察紀錄結果顯示肥大細胞含量之變化，以統計分析比較各組結果 (P<0.0167) 是具有顯著差異。前後兩次之一般全身血液生化及血球分析和寄生蟲檢驗均無明顯差異性，所以在治療實驗安全性評估結果為極安全的。
此研究提供給臨床獸醫師快速診斷犬異位性皮膚炎的參考，以及提供減敏療法治療犬異位性皮膚是一個有效且安全的證據。

關鍵字

犬 ； 異位性皮膚炎 ； 減敏針 ； 肥大細胞

並列摘要

Stubborn canine atopic dermatitis is a problem for veterinarians dealt with diagnosis and treatment. Therefore, easy diagnosis and effective and safe treatment of canine atopic dermatitis should be found for clinician. This research took place in from October, 2007 to June, 2008. Twenty-four dogs were randomly divided into three groups. The first group is the non-atopic controls; The second group is atopic dogs without treatment; The third group is the atopic dogs treated with allergen-specific immunotherapy (ASIT). When the experiment began, all dogs were analyzed for the concentration of IgG, IgE and the special allergy IgE in the analytic serum and they were also checked for general health, included blood biochemistry, blood cell analysis and parasite examination. In the meantime, skin biopsy was checked by histopathology. The third group was given the allergen-specific immunotherapy treatment. Every month all dogs were drawn blood for determination of IgG, IgE in the analytic serum for consecutive 9 months. The date of this experiment were calculated and analyzed by nonparametrical analysis. In serology, the statistical analysis results of IgG and IgE concentration among all groups did not appear remarkable difference (P>0.0167). According to the record of histopathologic studies, found that the difference of mast cell, number was smaller by 0.0167 in comparison to before immunotherapy P value, which had remarkable difference. It was considered an effective reference in the diagnosis and the treatment of atopic dermatitis. There was no significant difference on both blood biochemistry, blood cell analysis and parasite examination among groups. Therefore, it was very safe for allergen-specific immunotherapy.
The results of research provide reference for clinicians to diagnosis atopic dermatitis rapidly. And they providing treatment of atopic dermatitis with allergen-specific immunotherapy are efficient and safe approach.

並列關鍵字

dog. ； atopic dermatitis ； allergen-specific immunotherapy (ASIT) ； IgG ； IgE ； mast cell

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