多醣體攝入對白蝦(Litopenaeus vannamei)免疫相關基因表現的影響

Adjusted immune related genes expression in white shrimp (Litopenaeus vannamei) fed with polysaccharide

武德維(Vo-Duc Duy)

指導教授 ： 黃卓治

國立屏東科技大學/農學院/生物科技研究所/碩士(2013年)

https://doi.org/10.6346/NPUST.2013.00287

摘要

多醣可能刺激病原體相關分子模式（PAMPs）引發先天免疫反應。本研究設計5個實驗的飼料配方，每池200隻白蝦（初始重量0.84 G）飼養2個月。研究發現，含多醣飼料0.2，0.4，0.6和0.8克/公千，蝦的增重情形分別為669.58％，812.58％，774.25％，740.70％和707.21％（P<0.05）。定量PCR分析發現，供給多醣者在第20、第40和第60天培養之白蝦，在血細胞、hepatopancrea、鰓、肌肉和胃中之LvToll2，和Lvtoll3和γ干擾素誘導的溶酶體酶的巰基（Lv GILT）mRNA表達明顯上調，另一項實驗中，白蝦餵食含有多醣類的飼料後60天培養，105菌落（CFU）V. alginolyticus感染白蝦，然後放置在25‰海水飼養，飼餵含0.2和0.4g/公斤飼料11天後，餵食含有多醣類的飼料者，存活率顯著增加。因此，可以得出結論，餵食含有0.2和0.4g/公斤多醣類的飼料，可以提高白蝦的免疫能力，增加其抵抗V. alginolyticus感染之能力。

關鍵字

白蝦(Litopenaeus vannamei) ； 白蝦(Litopenaeus vannamei) ； V. alginolyticus ； 多醣 ； Toll樣受體 ； 干擾素 γ

並列摘要

Polysaccharides may contain pathogen-associated moleculer patterns (PAMPS) stimulated innate immune response in white shrimp (Litopenaeus vannamei). There are total of 5 experimental diets, each was fed to 200 shrimps per tank (initial wt: 0.84 g) for 2 months. Weight gain of shrimp fed control diet, 0.2, 0.4 and 0.6 and 0.8 g kg-1 polysaccharide containing diet increased 669.58 %, 812.58 %, 774.25%, 740.70% and 707.21% (p<0.05), respectively. Quantitative PCR analysis showed that expression mRNA level of LvToll 2, and Lvtoll 3 and interferon gamma inducible lysosomal thiol enzyme (Lv GILT) was clearly up-regulated in hemocyte, hepatopancrea, gill, muscle and stomach of both pellet feeding supplemented with polysaccharide at 20th, 40th and 60th day culturing, compared to shrimp fed control diet. In another experiment, L. vannamei, which had been fed control diet, or polysaccharide-containing diets after 60 days culturing, were challenged with V. alginolyticus at 105 colony-forming units (CFU) shrimp-1 and then placed in sea water of 25‰. The survival of shrimp fed a diet containing 0.2 and 0.4 g kg -1 after 11 days injected increased significantly, as compared to that of shrimp fed control diet. It is therefore concluded that administration of polysaccharide in the diet at 0.2 and 0.4 g kg-1 could enhance the immune ability of L. vannamei and increase its resistance to V. alginolyticus infection

並列關鍵字

White shrimp (Litopenaeus vannamei) ； White shrimp (Litopenaeus vannamei) ； Vibrio alginolyticus ； Polysaccharide ； toll-like receptor ； interferon gamma inducible lysosomal thiol

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