評估靈芝多醣萃取物作為動物疫苗佐劑之效力

To evaluate the potency of Ganoderma lucidum polysaccharide extract as an animal vaccine adjuvant

蔡威顥(Xai Hao-Wei)

指導教授 ： 鄭力廷

國立屏東科技大學/獸醫學院/動物疫苗科技研究所/碩士(2018年)

https://doi.org/10.6346/THE.NPUST.AVT.002.2018.D03

摘要

靈芝自古以來就被認為能增強抵抗力，而現代的科學也已證實靈芝具有抗腫瘤及免疫調節的活性。靈芝多醣萃取物已被廣泛地研究為免疫調節劑且能引起很強的免疫反應。在本次研究中，將靈芝多醣萃取物作為動物疫苗佐劑，並搭配重組多殺性巴士德桿菌外膜脂蛋白 E (rPlpE) 作為增強此抗原之免疫原性的對象。以小鼠作為實驗模型進行 In vitro、In vivo 試驗。在 In vitro 結果中，靈芝多醣萃取物誘導小鼠巨噬細胞產生一氧化氮以及 Th1 細胞相關細胞激素 TFN-

關鍵字

靈芝多醣萃取物 ； 細胞激素 ； 一氧化氮

並列摘要

Ganoderma lucidum has since been considered to enhance resistance, and modern science has also confirmed that Ganoderma lucidum has anti-tumor and immune regulation of activity. Ganoderma lucidum polysaccharide extract (G. lucidum) has been extensively studied as an immunomodulator and it can cause a strong immune response. In this study, the use of G. lucidum as an animal vaccine adjuvant is expected to enhance the antigenicity of the recombinant bactericide Pasteurella lipoprotein E (rPlpE). In the In vitro results, G. lucidum induced nitric oxide and Th1 cell-associated cytokines TFN-α and IL-12 in mouse macrophages. In vivo results showed that on the 14th day and 21st day, the total IgG was significantly higher in the group of adding adjuvant than group of antigen alone (P<0.05). G. lucidum as an adjuvant for animal vaccines not only enhance the immunogenicity of antigens, but also provides host protection earlier.

並列關鍵字

Ganoderma lucidum ； cytokine ； nitric oxide

參考文獻

[1] Steinhagen F, Kinjo T, Bode C, Klinman DM. TLR-based immune adjuvants. Vaccine. 2011;29:3341-55.

[2] Sun LX, Lin ZB, Lu J, Li WD, Niu YD, Sun Y, et al. The improvement of M1 polarization in macrophages by glycopeptide derived from Ganoderma lucidum. Immunol Res. 2017.

[3] Li WJ, Tang XF, Shuai XX, Jiang CJ, Liu X, Wang LF, et al. Mannose Receptor Mediates the Immune Response to Ganoderma atrum Polysaccharides in Macrophages. J Agric Food Chem. 2017;65:348-57.

[4] Pellegrino P, Falvella FS, Cheli S, Perrotta C, Clementi E, Radice S. The role of Toll-like receptor 4 polymorphisms in vaccine immune response. Pharmacogenomics J. 2016;16:96-101.

[5] Liu Z, Xing J, Zheng S, Bo R, Luo L, Huang Y, et al. Ganoderma lucidum polysaccharides encapsulated in liposome as an adjuvant to promote Th1-bias immune response. Carbohydr Polym. 2016;142:141-8.