以酸驅動啟動子YGP1進行螢光酶表達之探討

Study of an acid-driven promoter YGP1 for luciferase enzyme expression

吳亭逸(Wu Ting-Yi)

指導教授 ： 郭嘉信

國立屏東科技大學/農學院/食品科學系所/碩士(2019年)

https://doi.org/10.6346/NPUST201900404

摘要

在發酵酒精的過程當中經常因為營養源的不足，導致酵母菌提前進入穩定期也誘發出的許多生理變化。這些而啟動子則會感知環境的變化調控基因的表達。啟動子在遺傳基因中扮演著重要角色，可以決定基因的活動，並控制細胞開始生產所需的蛋白質。當啟動子被誘發時，將會啟動基因調控生產所需蛋白質。本實驗的目的為對將具有酸誘導性YGP1啟動子和螢光素轉殖至質體pGL3當中，再將質體轉型至大腸桿菌中，建設具有調控機制之基因重組大腸桿菌。目前YGP1啟動子已由萃取BCRC21841及BCRC22752兩株酵母菌之DNA中利用聚合酶連鎖反應技術取得，經由基因定序確認序列正確性後，將會測試酸性誘導啟動子在不同pH值下誘導螢光素發光的情形。

關鍵字

YGP1 ； 啟動子 ； 酸誘導性 ； 轉殖 ； 聚合酶連鎖反應

並列摘要

Nutrient starvation in the yeast Saccharomyces cerevisiae leads to a number of physiological changes that accompany entry into stationary phase. The expression of genes whose products play a role in stress adaptation is regulated in a manner that allows the cell to sense and responds to changing environmental conditions. Promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA. This purpose of this experiment is construction a gene recombinant plasmid with gene regulation system by colony pH sensitive promoter and luciferaes reporter gene into E.coli. It has already obtained YGP1 gene, from BCRC21841and BCRC22752 DNA by using polymerase chain reaction (PCR) technique. After gene confirmation via sequencing, the recombinant E.coli will be constructed and investigate its luciferase situation in different pH

並列關鍵字

YGP1 ； promoter ； pH-inducible ； transformation ； polymerase chain reaction

參考文獻

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