液化澱粉芽孢桿菌胞外多醣活化腸道上皮細胞苦味接受器及葡萄糖吸收之研究

Study on the Activation of Bitter Taste Receptors and Glucose Consumption of Intestinal Epithelial Cells by the Exopolysaccharides of Bacillus amyloliquefaciens

杜雅欣(Diwyacitta Antya Putri)

指導教授 ： 鄭雪玲 教授

國立屏東科技大學/農學院/生物科技系/碩士(2018年)

https://doi.org/10.6346/THE.NPUST.BST.007.2018.D01

摘要

已經證明來自液化芽孢桿菌的胞外多醣 (Exopolysaccharides; EPS)具有降血糖作用。同時，EPS溶液味道苦，而苦味接受器的活化已被認為與血糖的調節有關。因此，本研究的目的是分析EPS的降血糖作用是否與苦味接受器（TAS2R）的活化有關。 NCI-H716和IEC-18細胞分別作為腸上皮內分泌細胞和腸上皮細胞的模型。以EPS處理NCI-H716細胞時，觀察到細胞內Ca2+濃度的增加；同時EPS也促進NCI-H716細胞分泌GLP-1。以EPS處理IEC-18細胞時，細胞的葡萄糖消耗明顯增加，且胰島素訊息傳遞路徑中的因子，AKT，被活化。這些結果支持EPS可能活化腸內分泌細胞上的TAS2R，導致GLP-1的分泌，GLP-1是一種促進胰島素分泌的激素。同時，EPS活化胰島素訊息傳遞路徑中的因子並增加腸上皮細胞的葡萄糖消耗。EPS的降血糖功能可能與 EPS對腸道細胞的這些作用有關。
關鍵詞：胞外多醣，液化澱粉芽孢桿菌，降血糖作用，苦味接收器，腸道細胞

關鍵字

胞外多醣 ； 液化澱粉芽孢桿菌 ； 降血糖作用 ； 苦味接收器 ； 腸道細胞

並列摘要

Exopolysaccharides (EPS) from Bacillus amyloliquefaciens have been demonstarted to have a hypoglycemic effect. Meanwhile, the EPS solution tastes bitter, while the activation of bitter taste receptors has been suggested to be associated with the regulation of blood glucose. Therefore, the purpose of this study is to characterize whether the hypoglycemic effect of EPS is associated with the activation of bitter taste receptors (TAS2R). NCI-H716, and IEC-18 cells were used as a model of the intestinal enteroendocrine cells, and that of intestinal epithelial cells, respectively. When NCI-H716 cells were treated with EPS, an increase in the intracellular Ca2+ concentration was observed. The secretion of GLP-1 from NCI-H716 cells was also promoted after EPS treatment. Meanwhile, when IEC-18 cells were treated with EPS, glucose consumption of the cells was obviously enhanced, and AKT, a signaling factor in the insulin-signaling pathway, was activated in the cells. These results supported that EPS might activate TAS2R in intestinal enteroendocrine cells, leading to the secretion of GLP-1, a hormone promoting the secretion of insulin. Meanwhile, EPS activated effectors in the insulin-signaling pathway and increased the glucose consumption of intestinal epithelial cells. These effects of EPS on the intestinal cells likely play roles in the hypoglycemic function of EPS.

Keywords : Exopolysaccharides, Bacillus amyloliquefaciens, hypoglycemic effect, bitter taste receptor, intestinal cells

並列關鍵字

Exopolysaccharides ； Bacillus amyloliquefaciens ； hypoglycemic effect ； bitter taste receptor ； intestinal cells

參考文獻

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