快速微流體混合晶片系統應用於己二烯酸鉀之檢測

本研究使用了一種集成的微流體實驗平台，並使用此平台去檢測己二烯酸。集成的微流體實驗平台包含了用於物種混合反應的混合晶片，檢測己二烯酸的微光譜檢測區域，此區域也包含了加熱反應。混合晶片使用低成本的基材PMMA，並在上面使用CO2雷射加工形成圖形，並使用熱壓接合技術進行晶片的封裝。在所提出的平台之中將己二烯酸樣品與硫酸銨鐵Iron(III)透過氣體壓力驅動進行混合，然後透過集成的微流體實驗平台進行硫代巴比妥酸TBA的呈色反應，並使用微型光譜儀於530nm波長下偵測其吸光值，並拉出檢量線值用以確定己二烯酸的濃度。實驗結果，使用已知濃度範圍為0.02 g/kg~0.5 g/kg的己二烯酸，線性回歸值R2有0.995以上的線性度。並使用二種市售樣品實際檢測來驗證樣品中的己二烯酸濃度，藉此來證明微流體實驗平台的系統有效性。

關鍵字

己二烯酸；微流體；微型光譜儀；混合

並列摘要

An integrated microfluidic experimental platform is presented for sorbic acid (C6H8O2) concentration detection purposes.The integrated microfluidic platform comprises a mixer and heater zone for species mixing / reaction and a micro-spectrometer system detection zone for C6H8O2 concentration detection. The microfluidic reaction chip is patterned on low-cost PMMA substrates using a commercial CO2 laser system and is packaged using a hot-press bonding technique. In the proposed platform, the C6H8O2 sample and Iron(III) reagent are mixed under the effects of a high-pressure gas driving force. A colorimetric reaction (oxidation reaction) of the mixed species is then induced by means of an integrated micro heating / cooling system. The microfluidic reaction chip is then transferred to a micro-spectrometer system for detection purposes. Absorbance was measured at 530 nm using a micro-spectrometer wherein a line regression is obtained to determine the C6H8O2 concentration. The experimental results show that correlation coefficients of R2 = 0.995 is obtained with known concentrations ranging from 0.02 g/kg~0.5 g/kg. The real-world applicability of the proposed experimental platform is demonstrated by measuring the C6H8O2 concentration in two commercial food samples.