戊糖乳酸菌BD6 (Lactobacillus pentosus BD6) 添加於飼料對白對蝦成長及健康狀況的影響

Effects of Lactobacillus pentosus BD6 supplemented in diet on the growth, health status of white shrimp, Litopenaeus vannamei

杜翰(Tohap Simangunsong)

指導教授 ： 劉俊宏

國立屏東科技大學/農學院/水產養殖系所/碩士(2019年)

https://doi.org/10.6346/NPUST202000026

摘要

摘要
學號：M10613012
論文名稱：戊糖乳酸菌BD6 (Lactobacillus pentosus BD6) 添加於飼料對白對蝦成長及健康狀況的影響
總頁數：81頁
學校名稱：國立屏東科技大學 系 (所)別：水產養殖系
畢業時間及摘要別：一零八學年度第二學期碩士學位論文摘要
研究生：杜翰 指導教授：劉俊宏博士
論文摘要內容：
本研究目的為評估不同濃度之戊糖乳酸菌BD6添加於飼料中對南美白對蝦之成長、抗病力及免疫的影響。實驗結果顯示，戊糖乳酸菌BD6可有效抑制水產病原，其抑制圈大小介於1.7 ± 0.15公分至2.8 ± 0.12 公分。實驗蝦隻平均體重為2.56 ± 0.273 公克，實驗組別包括：控制組和添加有107、108、109及1010 CFU kg-1戊糖乳酸菌BD6的飼料，共計5組，每組三重覆，進行60天的餵養。實驗結果顯示，餵飼添加109及1010 CFU kg-1戊糖乳酸菌BD6組的飼料白蝦與控制組相比，其末重、增重率、特定生長率及飼料效益顯著增加。整體而言，攝食添加107及108 CFU kg-1戊糖乳酸菌BD6組別飼料的白蝦在成長上與控制組相比無顯著差異。以溶藻弧菌進行攻毒試驗後，飼料中添加1010 CFU kg-1戊糖乳酸菌BD6組之存活率較其他處理組高。免疫分析顯示，溶菌酶活性 (lysozyme)以攝食添加109 CFU kg-1戊糖乳酸菌BD6組之白蝦有顯著增加；吞噬活性 (PA)為攝食添加108 CFU kg-1戊糖乳酸菌BD6組之白蝦顯著提高，且與控制組相比提高兩倍；但超氧化物歧化酶 (SOD)、呼吸爆 (RBs)、總血球數 (THC)及酚氧化酶 (PO)等活性在控制組與添加戊糖乳酸菌BD6組之間均無顯著差異。而在白蝦肝胰腺和血球細胞中的LGBP、proPO I、proPO II及PEN4基因的表現部分，控制組與實驗組之間均無顯著差異。綜觀而言，白蝦飼料中添加戊糖乳酸菌BD6能夠有效改善蝦隻成長表現、提高對溶藻弧菌的抵抗力及免疫力。

關鍵字：白蝦、益生菌、戊糖乳酸菌BD6、抗菌活性、成長表現、免疫反應、免疫相關基因

關鍵字

白蝦 ； 益生菌 ； 戊糖乳酸菌BD6 ； 抗菌活性 ； 成長表現 ； 免疫反應 ； 免疫相關基因

並列摘要

Abstract
Student ID: M10613012
Title of Thesis: Effects of Lactobacillus pentosus BD6 supplemented in diet on the growth, health status of white shrimp, Litopenaeus vannamei
Total page: 81 pages
Name of Institute: Department of Aquaculture, National Pingtung University of Science and Technology
Graduate Date: 26 December, 2019 Degree Conferred: Master
Name of Student: Tohap Simangunsong Advisor: Chun-Hung Liu, Ph. D
The Contents of Abstract in This Thesis:
This study evaluates the administration of Lactobacillus pentosus BD6 strain as a probiotic with different doses on growth, disease resistance, and immunity of white shrimp, Litopenaeus vannamei. Lac. pentosus BD6 strains are effective in inhibiting the growth of all tested aquatic pathogens with the diameter of the inhibition zone appearing in the range of 1.7 ± 0.15 and 2.8 ± 0.12 cm. Shrimp (mean weight 2.56 ± 0.27 g) were fed for 60 days with diet control and treatment with probiotics of at 107, 108, 109, and 1010 CFU kg-1 and each group was carried out in triplicate. Diet with Lac. pentosus BD6 at doses 109 and 1010 CFU kg-1 significantly increases final weight, percentage weight gain, specific growth rate, and feed efficiency when compared to control. After a challenge test with Vibrio alginolyticus for four days, shrimp fed a diet at a dose of 1010 CFU kg-1 had a higher survival rate compared to other treatments and control. Supplementation with Lac. pentosus BD6 at a dose of 109 CFU kg-1 significantly increased lysozyme (LYS) activity on white shrimp. While shrimp supplemented with Lac. pentosus BD6 at a dose of 108 CFU kg-1 significantly increased phagocytic activity (PA) of L. vannamei. It was found that PA in shrimp given 108 CFU kg-1 increased twofold compared to control. However, there were no significant differences in superoxide dismutase (SOD), respiratory burst (RBs), total hemocyte count (THC), and phenoloxidase (PO) activity among control and all treatments. No significant differences in the expression level of LGBP, proPO I, proPO II, and PEN4 in hepatopancreas and hematocytes of shrimp were observed among all treatments and control. It was concluded that dietary supplementation of Lac. pentosus BD6 can be used in improving growth performance, disease resistance against V. alginolyticus infection, and immunity of L. vannamei through diet.
Keyword: L. vannamei, probiotic, Lac. pentosus BD6, antibacterial activity, growth performance, immune response, immune-related genes

並列關鍵字

L. vannamei ； probiotic ； Lac. pentosus BD6 ； antibacterial activity ； growth performance ； immune response ； immune-related genes

參考文獻

REFERENCES

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