兔腸道益生菌的篩選與活性分析

市售發酵製品等相關的益生菌產品常見添加菌種有嗜酸乳桿菌 (Lactobacillus acidophilus)、乾酪乳桿菌 (Lactobacillus casei)、副乾酪乳桿菌 (Lactobacillus paracasei)、保加利亞乳酸桿菌 (Lactobacillus bulgaricus)、比菲德氏菌 (Bifidobacterium spp.)、嗜熱鏈球菌(Streptococcus thermophilus)、植物乳桿菌 (Lactiplantibacillus plantarum) 等。益生菌對宿主來體內的免疫系統來說是外來物質，所以能否適應速主體內腸胃道環境對益生菌來說是一大考驗，若對胃酸及膽鹽的耐受性極低那益生菌也沒辦法順利到達後腸被吸收，也達不到促進及改善健康的效果，因此益生菌篩選條件主要挑選能適應腸胃道環境、存活率佳的菌株，再針對其他益生菌篩選標準去做試驗分析。本實驗選出7株已鑑定完畢的菌株，其中兩株作為對照組比對用，模擬腸胃道環境做了耐酸性、耐膽鹽的存活率試驗，還有測定生長曲線、溶血性試驗、真空冷凍乾燥存活率、活性測試定性及定量 (CMC分解酶、Xylan分解酶、Pectin分解酶、Starch分解酶、Protein分解酶)、吸附性試驗 (疏水性、自動聚合) 等，來綜合評估各個菌株的可開發性、應用方面及後續開發潛能。結果為，LP5、LP19、PTA22、LP1、LP2有些微α-溶血，PAL44、SL45並無觀察到溶血；冷凍乾燥存活率為PAL44最佳；耐酸性試驗LP5、LP19、PTA22均表現出良好耐酸性；耐膽鹽試驗PAL44、SL45表現良好耐膽鹽性；活性分析試驗在CMCase、Xylanase、Pectinase、Protease中LP5、LP19、PTA22、LP1、LP2均有表現出活性，在Amylase中PAL44、SL45有表現出活性；在吸附性試驗的疏水性試驗中，PAL44表現出最高疏水性；在自動聚合試驗中PAL44、SL45表現較佳，且7株菌的自動聚合率都有隨著時間增加。實驗過後決定將PTA22列入開發菌株；PAL44、SL45活性分析不佳，但有文獻指出可朝海洋生物益生菌及合成金銀奈米粒子的方面去做研究分析。

關鍵字

益生菌；乳酸菌；活性分析

並列摘要

Commercially available fermented products and other related probiotic products commonly added bacteria include Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus bulgaricus, Bifidobacterium spp., Streptococcus thermophilus, Lactiplantibacillus plantarum, etc. Probiotics are foreign substances to the host’s immune system, so whether they can adapt to the gastrointestinal environment of the main body is a big test for probiotics. If the tolerance to gastric acid and bile salts is extremely low, then probiotics There is no way to smoothly reach the hindgut to be absorbed, and the effect of promoting and improving health cannot be achieved. Therefore, the probiotic screening conditions are mainly to select strains that can adapt to the gastrointestinal environment and have good survival rates, and then test against other probiotic screening standards. analyze. This experiment selected 7 strains that have been identified, two of which were used as a control group to simulate the gastrointestinal tract environment for acid resistance and Bile salt survival test, as well as growth curve determination, hemolysis test, and vacuum freezing. Dry survival rate, activity test qualitative and quantitative (CMCase activity test, xylanase activity test, pectinase activity test, amylase activity test, protease activity test), adsorption test (hydrophobicity test、auto-aggregation), etc., to comprehensively evaluate the viability of each strain development, application and subsequent development potential. The results are that LP5, LP19, PTA22, LP1, LP2 have some slight α-hemolysis, and PAL44 and SL45 have no hemolysis; freeze-drying survival rate is the best for PAL44; acid resistance test LP5, LP19, PTA22 all show good acid resistance ; bile salt tolerance test PAL44, SL45 showed good bile salt tolerance; activity analysis test showed activity in CMCase, xylanase, pectinase, protease, LP5, LP19, PTA22, LP1, LP2, and PAL44, SL45 in amylase In the hydrophobicity test of the adsorption test, PAL44 showed the highest hydrophobicity; in the automatic polymerization test, PAL44 and SL45 performed better, and the automatic polymerization rate of the 7 strains increased with time. After the experiment, it was decided to include PTA22 as the developed strain; PAL44 and SL45 activity analysis was not good, but someliterature pointed out that research and analysis could be done in terms of marine probiotics and synthetic gold and silver nanoparticles.

並列關鍵字

Probiotics ； Lactic acid bacteria ； Activity analysis

參考文獻

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