苦苣苔科之種間與屬間授粉及雜交後裔性狀之研究

Study on Interspecific and Intergeneric Pollinations and Hybrid Offsprings in Gesneriaceae

馮思華(Francoise Jean Baptiste)

指導教授 ： 方中宜

國立屏東科技大學/國際學院/熱帶農業暨國際合作系/碩士(2021年)

https://doi.org/10.6346/NPUST202100422

摘要

苦苣苔科植物因其多樣、顯眼和吸引人的花葉圖案而被以各種創新方式用於室內外景觀。 非洲菫屬(Saintpaulia)和岩桐屬(Sinningia)是苦苣苔科中廣泛在市場上流通和最具知名的兩屬植物，其他屬則較不被認識和流通性少，開發具有更強抗蟲和抗病性、耐高/低溫性、更佳的開花特性和更高觀賞價值的新雜交品種是極具吸引力的。新雜交品種可以通過雜交授粉產生，但前提是花粉和柱頭在授粉時是具有活性和感受性的，有關苦苣苔科雜交授粉的科學研究非常有限，因此，本研究旨在定義授粉時間，進行種間和屬間雜交，並評估雜交植物的形態。 本研究使用九屬共19 種苦苣苔科植物，包括 Achimenes flava、 Alsobia ‘Cygnet’、Deinostigma eberhardtii、Deinostigma tamiana、Episcia ‘Blue Nile’、Episcia ‘Jim’s Canadian Sunset’、Episcia ‘Lil Lemon’、Episcia ‘Silver Skies’、Episcia ‘Thad’s Yellow Bird’, Episcia ‘Tiger Stripe’, Kohleria ‘Brimstone’, Kohleria ‘HCY’s Sevinalla’, Saintpaulia ‘Lyon’s Party Parasol’, Sinningia ‘HCY’s Peach Fragrance’, Sinningia ‘Mark Twain’, Smithiantha ‘An’s Hanabi’, Smithiantha ‘Petite Chérie’，Smithiantha salmon hybrid 和，Stretocarpella saxorum。首先，通過在含有不同濃度蔗糖（10、20 或 30%）、硼酸（50、100、200 或 400 毫克/升）和硝酸鈣（200 或 400 毫克/升）的培養基上進行體外花粉萌發試驗，研究結果顯示，含有 10% 蔗糖的培養基優於含 20% 和 30% 蔗糖的培養基，硼酸在 50 毫克/升的劑量下顯著增加花粉萌發和花粉管生長，硝酸鈣在 200 或 400 毫克/升時對花粉萌發和花粉管生長有效，因此選擇含有10%蔗糖、50 毫克/升硼酸和200毫克/升硝酸鈣的培養基來進行後續實驗。從開花到花衰每日評估花粉活力，發現花粉採集的最佳時機為開花後第1天。基於雌蕊長度和柱頭形態分析柱頭的感受性，發現依據測試植物的不同，在開花後 3 至 20 天有較高的柱頭感受性，最常柱頭長度為2.03-3.50公分，成熟的柱頭比不成熟的柱頭有更好的柱頭感受性。通過人工授粉進行種間和屬間雜交，從 105個雜交組合中獲得了 11 個成功的種間雜交（50%）和 13個屬間雜交（15.6%），種間雜交 (36.3%) 比屬間雜交 (3.6%) 產生更多有活性的種子，目前針對十四種具有潛力的種間和屬間雜種進行外貌形態和市場潛力評估。

關鍵字

苦苣苔科 ； 雜種種子 ； 屬間雜種 ； 種間雜種 ； 花粉活力 ； 授粉時間

並列摘要

Gesneriaceae plants have been used in a variety of innovative ways in landscaping and indoors because of their diverse, conspicuous, attractive flower and leaf patterns. Plants of the genera Saintpaulia and Sinningia are widely commercialized in the market and are the most known of the family Gesneriaceae. Plants of the other genera are less popular and not readily available commercially. There is a high interest to develop new hybrids with improved resistance to pests, diseases and high/low temperatures, better blooming properties, or enhanced ornamental values. New hybrids can be created via cross pollination, which requires that the pollens are viable and stigmas are receptive at the time of the crosses. However, scientific studies on cross pollination are very limited in this family. Hence, this study was aimed to define the timing for pollination, conduct interspecific and intergeneric crosses, and evaluating the morphology of the hybrid plants. This study made use of nineteen gesneriad plants from nine genera, including Achimenes flava, Alsobia ‘Cygnet’, Deinostigma eberhardtii, Deinostigma tamiana, Episcia ‘Blue Nile’, Episcia ‘Jim’s Canadian Sunset’, Episcia ‘Lil Lemon’, Episcia ‘Silver Skies’, Episcia ‘Thad’s Yellow Bird’, Episcia ‘Tiger Stripe’, Kohleria ‘Brimstone’, Kohleria ‘HCY’s Sevinalla’, Saintpaulia ‘Lyon’s Party Parasol’, Sinningia ‘HCY’s Peach Fragrance’, Sinningia ‘Mark Twain’, Smithiantha ‘An’s Hanabi’, Smithiantha ‘Petite Chérie’, Smithiantha salmon hybrid, Stretocarpella saxorum. First, in vitro pollen germination on medium enriched with varied amounts of sucrose (10%, 20%, or 30%), boric acid (50, 100, 200, or 400 mg/l), and calcium nitrate (200 or 400 mg/l) was used to assess pollen viability. The results showed that media containing 10% sucrose outperformed media with 20 and 30% sucrose. Boric acid, at a dosage of 50 mg/l, significantly enhanced pollen germination and pollen tube length. At 200 or 400 mg/l, calcium nitrate proved helpful in promoting pollen germination and pollen tube growth. Later studies were carried out using a medium with 10% sucrose, 50 mg/l boric acid, and 200 mg/l calcium nitrate. The viability of pollens from anthesis to flower senescence was evaluated daily and it was found that the optimum time for collecting pollens was at day 1 after anthesis. The receptivity of stigma was analyzed based on pistil length and stigma morphology. The longest pistil length was observed from 3 to 20 days after anthesis depending on the plant tested varied from 2.03 to 3.50 cm. Mature stigmas appeared to be more receptive than immature ones. Interspecific and intergeneric crosses were performed by hand pollination. A total of 105 cross combinations were made from which eleven successful interspecific crosses (50%) and thirteen intergeneric crosses (15.6%) were obtained. Interspecific crosses (36.3%) produced more viable seeds than intergeneric crosses (3.6%). Fourteen interspecific and intergeneric hybrids of interest are undergoing morphological and market potential evaluations.

並列關鍵字

Gesneriaceae ； hybrid seed ； intergeneric hybrid ； interspecific hybrid ； pollen viability ； pollination time

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