探討碳基奈米材料毒性對於秀麗線蟲之發展影響

奈米製程的產品應用於許多領域，但製造、使用與回收過程皆有釋放至環境的疑慮存在，因此有進行奈米顆粒對於生物體之毒性影響的必要。本研究所使用的動物模型為秀麗線蟲（Caenorhabditis elegans, C.elegans），並透過多項毒性試驗來探討長時間暴露對其之毒性影響。首先本研究暴露氧化石墨烯（graphene oxide, GO）與控制組相比，暴露GO所有濃度之線蟲致死率無顯著差異，但會顯著降低秀麗線蟲頭部擺動與身體彎曲、產卵數量、體長與壽命。另外RT-PCR結果顯示暴露較高濃度之GO會顯著增加抗氧化劑相關的基因如sod-1、sod-3與ctl-2 mRNA的表現，透果此結果可看到長時間暴露在低濃度GO下仍會影響秀麗線蟲的神經系統與生殖系統功能、縮短體長與壽命並造成氧化壓力。另外Titanium dioxide (TiO2)使用市售與人工合成之2種型式進行實驗，與控制組相比，2種TiO2對秀麗線蟲致死率無顯著影響，但暴露2種TiO2皆會顯著減少秀麗線蟲頭部擺動、身體彎曲、產卵數量與體長，會對神經系統與生殖系統造成影響。然而，與控制組相比，暴露所有濃度之市售TiO2對於秀麗線蟲壽命無顯著影響。未來本研究將繼續更深入進行不同面向的實驗，研究秀麗線蟲的毒理作用，從而評估奈米外料對於人體之毒性影響。關鍵詞：秀麗線蟲 ; 奈米材料 ; 氧化石墨烯 ; 二氧化鈦 ; 運動行為評估

關鍵字

秀麗線蟲；奈米材料；氧化石墨烯；二氧化鈦；運動行為評估

並列摘要

The engineered nanomaterials (ENMs) are utilized for industrial, biomedical, and environmental purposes, however, the environmental release during manufacture, transport, use, and disposal is still in doubt. The toxic effects of ENMs are also still lacking. The current study was aim to investigate the possible toxicity of the different ENMs（graphene oxide (GO) and Titanium dioxide (TiO2)）on an in-vivo model of Caenorhabditis elegans (C. elegans). The different toxicological parameter of C. elegans were investigated, including lethality (Lethality Assay), Length (Growth Measurement) reproductive ability (Reproductive), exercise ability (Locomotion), lifespan (Lifespan) and gene (Gene expression). In comparison with control, there was no significant difference in the lethality in all concentrations of GO exposure. But GO exposure significant decreased the head trashing number, body-bending number, brood size, body length, and lifespan of C. elegans. Real-time PCR analysis also indicated that higher concentration of GO significant increased the antioxidant-related genes sod-1, sod-3 and ctl-2 mRNA expression. In addition, low concentrations and long-term exposure of GO also affect the function of the C. elegans nervous system and reproductive system, shorten the length of the nematode body, life span, and cause oxidative stress. The TiO2 was used in current study including commercially available and synthetic forms similar to GO, both types of TiO2 exposure had no significant difference in lethality of C. elegans as compared with the control. Furthermore, both types of TiO2 exposure also significant decreased the head trashing number, and body-bending number, brood size, body length, and affect nervous system and reproductive system of C. elegans. However, all concentrations of commercially available TiO2 have no significant effect on the lifespan of C. elegans as compared with the control group. In the future, we will continue to conduct more in-depth experiments to investigate the toxic effects of C. elegans, leading to evaluate the toxic effects of the nanomaterials on the human body. Keywords：C.elegans ; Nanomayreial ; Graphene oxide ; Titanium dioxide ; Locomotion

並列關鍵字

C.elegans ； Nanomayreial ； Graphene oxide ； Titanium dioxide ； Locomotion

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