飼料中添加單寧酸對雞與豬糞便菌相之影響

Effects of dietary tannic acid on fecal microbiota of chicken and swine

楊希平(Yang Hsi-Ping)

指導教授 ： 彭劭于 ； 吳羽婷

國立屏東科技大學/農學院/動物科學與畜產系所/碩士(2022年)

https://doi.org/10.6346/NPUST202200448

摘要

單寧酸的抗菌及抗氧化特性使其成為取代抗生素使用及減少細菌產生抗藥性的潛在方案，本篇研究重點為單寧酸作為飼料添加劑對雞隻及豬隻糞便菌相之影響，實驗過程皆使用禽畜糞便進行16S rRNA萃取來獲得活菌菌相之結果用以推斷在腸道中仍存活且具有功能之菌種。結果顯示單寧酸具有調控菌群之能力，對雞隻糞便中的厚壁菌門(Firmicutes)有巨大影響力，在飼糧中添加0.1%單寧酸對雞隻及豬隻腸道中部分潛在致病菌群普雷沃氏菌科(Prevotellaceae)具抑制效果；無添加單寧酸的組別中以消化鏈球菌科(Peptostreptococcaceae)之菌群、螺桿菌屬(Helicobacter)、乳桿菌屬(Lactobacillus)及Clostridium sensu stricto 1為優勢菌群；添加單寧酸組別則以擬桿菌屬(Bacteroides)、螺桿菌屬(Helicobacter)、Akkermansia、Alistipes、瘤胃梭菌屬(Ruminiclostridium 9)及副薩特氏菌屬(Parasutterella)等細菌屬為優勢菌群。總而言之，單寧酸展現出調控菌群的能力，但部分革蘭氏陽性菌及非致病性菌群亦可能被抑制，除了對動物生長性能的影響外，菌群變化與宿主健康狀況的交互作用仍需深入探討，添加劑量與使用時機可能為其作為飼料添加劑是否具有明顯助益的關鍵。

關鍵字

腸道菌相 ； 糞便 ； 飼料添加劑 ； RNA萃取 ； 單寧酸

並列摘要

The antibacterial and antioxidant properties of tannic acid make it a potential solution to replace the use of antibiotics and reduce bacterial resistance. This study focuses on the effects of tannic acid as a feed additive on the fecal bacterial microbiota of chicken and swine. 16S rRNA extraction from animal feces was used to obtain the results of viable bacterial microbiota to infer the viable and functional bacteria species in the gut. The results showed that tannic acid had the ability to regulate the micriobiota, and had a great influence on Firmicutes in the feces of chicken. Dietary supplemented with 0.1% tannic acid had inhibitory effect on Prevotellaceae, a part of potential pathogenic bacteria group in the gut of chicken and swine. In the group without tannic acid, Peptostreptococcaceae, Helicobacter, Lactobacillus and Clostridium sensu stricto 1 were the dominant bacteria. The tannic acid groups were dominated by Bacteroides, Helicobacter, Akkermansia, Alistipes, Ruminiclostridium 9 and Parasutterella. In a nutshell, tannic acid showed the ability to control bacteria communities, but some gram-positive bacteria and non-pathogenic bacteria group could also be suppressed, in addition to the impact on animal growth performance, the interaction between microbiota changes and host health requires further discussion, add dose and when to use may be the key whether feed additive has obvious benefits.

並列關鍵字

Bacterial microbiota ； Feces ； Feed additives ； RNA extraction ； Tannic acid

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