丁香及肉桂精油抑制細菌生物膜活性之研究

Study on the Inhibitory Activity of Clove and Cinnamon Essential Oils on Bacterial Biofilms

謝華江(Shieh Hwa-Jiang-)

指導教授 ： 施玟玲

國立屏東科技大學/農學院/食品生技碩士學位學程在職專班/碩士(2022年)

https://doi.org/10.6346/NPUST202200159

摘要

目前為止發現了某些細菌對大多數常規抗生素具有抗藥性，研究指出細菌生物膜 (biofilm) 對多種抗生素具有高度抗性，通常難以通過抗生素治療生物膜感染疾病。因此，研發新穎抗菌劑用於治療生物膜感染極為重要。精油成分主要是次級代謝產物，它們是從植物中萃取的揮發性芳香產品。據研究顯示精油具有多種生物活性，包括抗氧化、抗菌、抗癌和抗發炎作用。精油是最具代表性的天然抗菌劑之一，多項研究已證明從植物中萃取的精油得以作為抗菌劑。本研究旨在分析丁香精油 (Clove essential oil) 及肉桂精油 (Cinnamon essential oil) 抑制細菌生物膜活性功能影響。丁香及肉桂精油之成分分析是透過使用氣相層析質譜儀 (Gas Chromatography-Mass Spectrometry, GC-MS) 分析兩精油所含之主要化合物種類及其含量百分比。精油抑制細菌生物膜實驗部分是使用生物膜根除試驗 (Minimum Biofilm Eradication Concentration Assay, MBEC Assay) 評估丁香精油及肉桂精油對於金黃色葡萄球菌 (Staphylococcus aureus, S. aureus) 及綠膿桿菌 (Pseudomonas aeruginosa, P. aeruginosa) 生物膜形成之影響。本研究透過於生物膜根除試驗結果證明丁香及肉桂精油對於S. aureus及P. aeruginosa兩菌株之生物膜形成皆有顯著之根除功效，並且對於該兩菌株細菌之生物膜最小根除濃度 (Minimum Biofilm Eradication Concentration, MBEC) 皆為 0.16 mg/mL。本研究結果顯示丁香與肉桂精油未來具有替代抗生素治療細菌生物膜感染疾病之潛力。

關鍵字

生物膜 ； 金黃色葡萄球菌 ； 丁香精油 ； 綠膿桿菌 ； 肉桂精油

並列摘要

Some bacteria have been found to be resistant to most conventional antibiotics so far, and studies have shown that bacterial biofilms are highly resistant to many antibiotics, and it is often difficult to treat biofilm-infected diseases with antibiotics. Therefore, the development of novel antibacterial agents for the treatment of biofilm infections is extremely important. Essential oil components are mainly secondary metabolites, which are volatile aromatic products extracted from plants. Essential oils have a variety of biological activities, including anti-oxidant, anti-bacterial, anti-cancer, and anti-inflammatory effects, according to research. Essential oils are one of the most representative natural antibacterial agents, and many studies have proved that essential oils extracted from plants can act as antibacterial agents. The purpose of this study was to analyze the functional effects of clove essential oil and cinnamon essential oil on inhibiting bacterial biofilm activity. The composition analysis of the essential oils of clove and cinnamon is by using Gas Chromatography-Mass Spectrometry (GC-MS) to analyze the main compounds and their content percentages in the two essential oils. Bacterial biofilm inhibition by essential oils was partly evaluated using the Minimum Biofilm Eradication Concentration Assay, MBEC Assay (MBEC Assay) to evaluate the effects of clove and cinnamon essential oils on Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) biofilms forming influence. In this study, the results of the biofilm eradication test proved that clove and cinnamon essential oils have significant eradication effects on the biofilm formation of S. aureus and P. aeruginosa strains, and the minimum biofilm eradication concentrations for the two strains of bacteria (Minimum Biofilm Eradication Concentration, MBEC) at 0.16 mg/mL. The results of this study show that clove and cinnamon essential oils have the potential to replace antibiotics in the treatment of bacterial biofilm infections in the future.

並列關鍵字

Biofilm ； Staphylococcus aureus ； clove essential oil ； Pseudomonas aeruginosa ； cinnamon essential oil

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