白藜蘆醇衍生物Resveratroloside對脂多醣誘導的RAW264.7細胞之抗發炎研究

Study of Anti-inflammatory Effects of Resveratrol Derivative Resveratroloside on Lipopolysaccharide-induced RAW264.7 Cells

張藝馨(Chang Yi-Hsin)

指導教授 ： 張誌益 ； 吳裕仁

國立屏東科技大學/農學院/生物科技系/碩士(2022年)

https://doi.org/10.6346/NPUST202200345

摘要

前人研究顯示白藜蘆醇的生物活性可能具有治療作用，像是抗氧化、抗發炎、預防心血管疾病、抗癌，這些天然活性物質具有開發成藥物的潛力。本研究探討白藜蘆醇的衍生物Resveratroloside於脂多醣(Lipopolysaccharide, LPS)所誘導的巨噬細胞RAW264.7中的抗發炎能力及其機制。經由MTT細胞存活率測試、一氧化氮(Nitric Oxide, NO)生成試驗、西方墨點法(Western blot)、免疫螢光染色(Immunofluorescence, IF)的實驗結果發現Resveratroloside對細胞毒性低且能抑制發炎反應物NO生成，並且抑制發炎相關蛋白質iNOS、COX-2之表現量。研究發現Resveratroloside透過抑制MAPK途徑及NF-κB磷酸化生成及轉位來阻斷發炎反應的進行。這些研究結果說明Resveratroloside的低細胞毒性及抗發炎能力具有作為抗炎藥物開發的潛能。

關鍵字

抗發炎 ； 白藜蘆醇衍生物 ； 脂多醣 ； 巨噬細胞 ； 西方墨點法 ； 免疫螢光

並列摘要

Previous studies have shown resveratrol possibly has biological activities for the treatment, such as antioxidant, anti-inflammatory, anti-cancer, cardiovascular disease prevention, and anti-cancer, which is potential in the drug. The purpose of this study was to investigate the anti-inflammatory activity and mechanism of resveratrol derivative Resveratroloside in Lipopolysaccharide (LPS) treated macrophage RAW264.7 cells. The results of MTT cell viability test, Nitric Oxide (NO) determination, Western blot, and Immunofluorescence (IF) showed that Resveratroloside has lower cytotoxicity and it can inhibit the production of nitric oxide, and also inhibit pro-inflammatory enzymes expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), that Resveratroloside reduces the inflammatory responses through inhibiting the MAPK signaling pathway and the phosphorylation and translocation of NF-κB. These data suggested that Resveratroloside has lower cytotoxicity and anti-inflammatory effect, which is potential for anti-inflammatory drug development.

並列關鍵字

anti-inflammatory ； resveratrol derivative ； LPS ； macrophage ； Western blot ； Immunofluorescence

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