利用糞便菌叢移植探討pterostilbene及其衍生物3'-hydroxypterostilbene對高脂飲食誘發肥胖小鼠腸道發炎與菌叢失調之影響

Effects of pterostilbene and its derivative 3'-hydroxypterostilbene on intestinal inflammation and dysbiosis in obesity mice induced by high-fat diet using fecal microbiota transplantation

楊佳芳(Yang Jia-fang)

指導教授 ： 陳與國

國立屏東科技大學/農學院/食品科學系所/碩士(2023年)

https://doi.org/10.6346/NPUST202300088

摘要

近年來，隨著飲食習慣逐漸西式化、精緻飲食增加及作息不正常，過多的能量逐漸堆積體內，導致脂肪組織的增生，使世界上罹患肥胖的人口正逐年上升，並且肥胖會提高其他併發症的發生機率，而根據研究指出，腸道中的腸道菌群之失調與人體的肥胖有一定的相關性。紫檀芪 (Pterostilbene, Pt) 和其衍生物3'-hydroxypterostilbene (OHPt) 是一種天然存在的芪類化合物，來源於多種天然植物，像是葡萄、花生及藍莓等，具有極高的生物活性，並在肥胖老鼠中有改善其腸道菌相的功用。故本研究為利用糞便菌叢移植模式探討Pt及其衍生物OHPt對於肥胖小鼠及腸道發炎反應的影響。本實驗組別總共分為7組：C組：基礎飲食；C-Pt組：基礎飲食+50 mg/kg/day Pt；C-OHPt組：C+50 mg/kg/day OHPt；HFD組：高脂飲食組；HFD-F-C組：高脂飲食組+糞便來自C組小鼠；HFD-F-Pt組：高脂飲食組+糞便來自C-Pt組小鼠；HFD-F-OHPt組：高脂飲食組+糞便來自C-OHPt組小鼠。實驗結果顯示，HFD-F-OHPt組小鼠血清中總膽固醇及三酸甘油酯含量明顯低於HFD-F-C組及HFD-F-Pt組，且在肝臟中脂質的累積也有明顯的降低，與HFD組相比具有顯著性差異 (p<0.05)。除此之外，在發炎指標性物質LPS方面亦具有降低發炎效果，並在西方墨點法中的數據顯示HFD-F-OHPt組降低發炎蛋白的表現優於HFD-F-C組及HFD-F-Pt組。根據以上結果可以得知，HFD-F-OHPt組的處理對於因肥胖而造成血管及肝臟的脂肪堆積，具有改善的效果，並能降低因肥胖而造成的發炎反應，且改善效果較優於HFD-F-C組及HFD-F-Pt組，後續實驗將會分析其糞便菌相，探討其改善肥胖之關鍵菌種，做為抗肥胖的益生質或益生菌保健食品之開發。

關鍵字

肥胖 ； 糞便菌叢移植 ； 菌叢失調 ； 腸道菌相

並列摘要

In recent years, with the gradual westernization of eating habits, exquisite diet and abnormal work and rest, too much energy gradually accumulates in the body, leading to the proliferation of adipose tissue, making the world's obese population increasing year by year, and obesity will increase the incidence of complications. According to the research, it is pointed out that the imbalance of intestinal flora in the intestine is related to obesity.
Red sandalwood stilbene and its derivative 3'- hydroxypterostilbene red sandalwood stilbene are natural stilbene compounds derived from a variety of natural plants, such as grapes, peanuts and blueberries. They have high bioavailability and can improve the intestinal microflora of obese mice. Therefore, the purpose of this study was to investigate the effects of pterostilbene and its derivatives on obese mice and intestinal inflammation by using the fecal flora transplantation model. The experimental group is divided into 7 groups：C group：basic diet, C-Pt group：basic diet +50 mg/kg/day pterostilbene, C-OHPt group：basic diet +50 mg/kg/day 3'-hydroxypterostilbene, HFD group：high-fat diet group, HFD-F-C group：high-fat diet group + feces from group C mice, HFD-F-Pt group：high-fat diet group + feces from group C-Pt mice, HFD-F-OHPt group：high-fat diet group +feces from group C-OHPt mice. According to the experimental results, the HFD-F-OHPt group decreased the total cholesterol and triglyceride in serum better than the HFD-F-C group and the HFD-F-Pt group, and significantly reduced the lipid accumulation in the liver. Compared with the HFD group, the HFD-F-OHPt group had a significant difference, and the inflammatory index substance LPS had a lower inflammatory effect. The data in the Western ink dot method showed that the HFD-F-OHPt group decreased the inflammatory response better than the HFD-F-C group and the HFD-F-C group. According to the above results, HFD-F-OHPt group can improve the fat accumulation in blood vessels and liver caused by obesity, and reduce the inflammatory reaction caused by obesity, and the improvement effect is better than that of HFD-F-Pt group and HFD-F-C group. Subsequent experiments will analyze the bacteria in feces, and explore the key bacteria to improve obesity, as a prebiotic or probiotic health food development for anti-obesity.

並列關鍵字

Obesity ； fecal microbiota transplantation ； dysbiosis ； gut microbiota

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