- 學位論文
超臨界流體與模擬移動床層析技術於五味子木酚素萃取與純化之應用
Application of supercritical fluid technology and simulated moving bed chromatography for the extraction and purification of lignans from Schisandra chinensis
摘要
本論文旨在針對五味子木酚素的萃取及分離進行探討。本論文首先利用超臨界二氧化碳萃取五味子果核中的五味子油，並探討萃取溫度、萃取壓力、以及輔溶劑濃度等操作條件對萃取率的影響，同時採用Sovova的萃取動力學模式進行實驗結果的擬合，以獲取萃取動力學的參數。本論文隨後利用實驗級的超臨界流體模擬移動床設備(SF-SMB)進行五味子木質酚分離，並藉由不同的操作條件，分別獲得五味子乙素、五味子醇甲，或者將五味子甲素與乙素以及五味子醇甲與醇乙加以以分離。有鑑於天然物分離從來沒有SF-SMB工業化放大量產經驗，本論文進一步利用生產級的SF-SMB進行五味子木質酚分離的放大測試，結果顯示依據實驗級設備所設定的操作條件便能夠簡單放大流量並在生產級設備上進行分離。在實驗級設備的研究過程中，發現大量存在的油脂使得純化所獲得的弱滯留五味子甲素與乙素純度偏低，因此本論文開發使用六區段的SMB來進行油脂、五味子甲素與乙素、以及五味子醇甲與醇乙的三成份分離方法，結果得到三種成分群的良好分離。本論文使用超臨界二氧化碳、乙醇、以及異丙醇進行五味子木酚素的萃取以及後續的分離純化，除了避免使用溶劑以外，所使用的溶劑及能源耗量小，將來一定能夠成為生產天然活性成份的一個綠色永續的分離純化方法。
關鍵字
none並列摘要
In this study, the lignans in the seed of Schisandra chinensis were extracted by supercritical fluid extraction (SFE), and subsequently separated by supercritical fluid simulated moving bed (SF-SMB). Effects of the extraction temperature, pressure and the content of ethanol as a cosolvent on the yield of seed oil were investigated. Sovova’s equation was applied to fit the experimental results to investigate the extraction kinetics.By setting different parameters, a lab-scale SF-SMB was employed to separateschisandrin B andschisandrol A from the extracted oil by SFE, or achieve theseparation of schisandrin A/Bandschisandrol A/B.Since natural product have never been produced by SF-SMB, this studyfirstly employed a pilot scale SF-SMB to study the scale-up for separating thelignans in the seed oil. As a result, the operating parameters obtained from the lab scale SF-SMB can simply and easily apply to conduct the separation at the pilot scale unit. During conducting the separtion of lignans, large amount of oil and fatresults inthe difficulties of obtaining high purity of Schisandrin A and Schisandrin B, becauseboth lignans have similar retention with the oil and fat in the supercritical fluid chromatography. A six-section SMB with liquid desorbentwas designed to separate the oil and fat, schisandrin A/B,and schisandrol A/B effectively.The proposed processes in this study used carbon dioxide, ethanol, and 2-propanol as the processing solvents for the extraction and separation of natural bioactive compounds to avoid the usage of harmful solvents, and saving solventand energy. The processwill become andgenerallybe recognized as a green and effective process for the production of bioactive ingredients.