Nursing staff are the first line of care for patients and reducing turnover is an important issue for healthcare managers in a situation where there is a shortage of nursing staff. The purpose of this study is to provide a reference for the hospital to formulate effective retention strategies by exploring the status and correlation of mindful awareness on job stress and turnover intention of hospital nursing staff, in order to reduce the turnover rate and maintain the quality of care. This survey follows a descriptive, correlational and inferential survey research design and was period from 2022/7/15 to 2022/09/30. Five hundred questionnaires were distributed to nursing staff at the regional hospital level and above in Kaohsiung and Ping-Tung areas and a total of 490 valid questionnaires were returned, with a valid return rate of 98%. The research data were analyzed with SPSS and AMOS statistical software. According to the results of the study, most of the research subjects were female, with an average age of 38.0 ± 9.8 years. The turnover intention of clinical nurses who were unmarried and childless at the age of 20 to 40 were significantly higher than that of those who were married and had 1 to 2 children over the age of 40, among level N0 nursing staff(work less than 1 year) than among level N3 nursing staff, among nursing staff with less than 10 years of total service, especially those with less than 5 years of service, than among those with more than 15 years of total service. Mindful awareness is statistically negatively correlated with job stress and turnover intention, that is, the more mindful the nursing staff are, the lower the job stress and turnover intention, the job stress and turnover intention is statistically positively correlated, that is, the higher their job stress, the higher their turnover intention. The results of the path analysis revealed that job stress can be reduced by mindful awareness. This study found that elderly people can also be engaged in nursing-related job and proper arrangement of nursing job may reduce the shortage of nursing manpower. After working in a high-stress environment, they still have to confront family problems when they return home. This is also one of the reasons for resignation. However, nearly 50% of the nursing staff did not have children, which is consistent with the current situation of low birth rate and is worth further discussion. Whether this is related to the pressure of family issues is also worth pondering. Effective retention of nursing staff has become the goal and challenge of hospital managers. In order to stabilize the nursing workforce, it is suggested to strengthen the friendly working environment and put forward effective retention strategies for the relevant factors of nursing staff's turnover intention.