假馬齒莧水萃物抑制肝星狀細胞活性及肝纖維化病鼠治療之評估

Evaluation of bacopa monnieri extracts on the inhibition of hepatic stellate cells and its therapeutic potential of liver fibrosis in rats

王昱雯(Yu-Wen Wang)

指導教授 ： 郭士民

義守大學/醫學科技學院/生物醫學工程學系/碩士(2022年)

摘要

人體肝臟損傷後體內的膠原纖維會增生以修復受損部位。而肝臟纖維化是由於肝臟內肝星狀細胞被激活後分泌大量膠原蛋白並過度沉積所導致，反覆或持續性的刺激會造成細胞外基質增生與代謝失衡。肝星狀細胞被激活的關鍵是氧化自由基的產生。近年，肝纖維化研究已發展可逆性的治療策略，認為阻止或延緩肝纖維化的發生即治癒多數慢性肝病。因此，降低肝星狀細胞的活化、增加肝星狀細胞凋亡、增加細胞外基質的分解以及減少細胞外基質的累積將是新的治療研究方向。假馬齒莧屬玄蔘科草本植物，含皂苷及醣苷等成分，具抗氧化、抗發炎、抗病毒、抗癌、免疫調節及酵素抑制等活性，且具止痛與清除自由基等能。
本論文欲以假馬齒莧萃取物抑制肝星狀細胞的活性並治療硫代乙醯胺(TAA)誘導的大鼠肝纖維化病鼠的評估。研究初步結果顯示：1.假馬齒莧可萃取皂苷成分。2.細胞型態及活/死染色結果顯示假馬齒莧 IC50 為 0.39 mg/mL。3.細胞凋亡分析假馬齒莧於 0.79 mg/mL 時細胞凋亡達 50%。4.H2O2誘導肝星狀細胞發炎而假馬齒莧可減緩發炎程度。5.TAA 誘導大鼠產生肝臟纖維化。後續將持續探討假馬齒莧萃取物改善肝損傷的作用。

關鍵字

肝纖維化 ； 假馬齒莧 ； 抗發炎 ； 肝星狀細胞 ； 水萃物

並列摘要

After the liver injury, collagen fibers will proliferate to repair the damaged area in vivo. Liver fibrosis is caused by the activation of hepatic stellate cells in the liver to secrete a large amount of collagen and excessive deposition. Repeated or
persistent stimulation will cause extracellular matrix proliferation and metabolic imbalance. The main factor for the activation of hepatic stellate cells is the production of oxidative free radicals. In recent years, reversible treatment strategies have been developed in the study of liver fibrosis. It is considered that
preventing or suspending the occurrence of liver fibrosis is the cure of most chronic liver diseases. Therefore, reducing the activation of hepatic stellate cells, increasing the apoptosis of hepatic stellate cells, increasing the decomposition of
extracellular matrix and reducing the accumulation of extracellular matrix will be a new therapeutic research direction. Bacopa monnieri is a herbaceous plant of the scrophulariaceae, containing saponins and glycosides, which have antioxidant,
anti-inflammatory, antiviral, anticancer, immune regulation and enzyme inhibition activities, as well as pain relief and free radical scavenging functions. The purpose of this master’s dissertation IV is evaluate the inhibition of hepatic stellate cell activity by Bacopa monnieri extract and the treatment of thioacetamide induced hepatic fibrosis in rats. The preliminary results show that: 1. Extraction of saponins. 2. Cell morphology and live/dead staining showed that the IC50 concentration of Bacopa monnieri was 0.39 mg/mL. 3. Apoptosis analysis the Bacopa monnieri reached 50% at 0.79 mg/mL. 4. Bacopa monnieri can reduce H2O2-induced hepatic stellate cell inflammation. 5. Induced liver fibrosis in experimental rats. The follow-up will continue to explore the effect of Bacopa monnieri on improving hepatic stellate cell inflammation after TAA stimulation in rats.

並列關鍵字

Liver fibrosis ； Bacopa monnieri ； anti-inflammatory ； hepatic stellate cells ； water extract

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