脫氧雪腐鐮刀菌烯醇毒素對凝結芽孢桿菌及芽孢桿菌生長之影響

The growths of Bacillus coagulans and Bacillus subtilis in the presence of deoxynivalenol

黃宣凱(Shiuan-kai Huang)

指導教授 ： 陳齊聖

朝陽科技大學/理工學院/應用化學系/碩士(2012年)

摘要

本研究目的為探討脫氧雪腐鐮刀菌烯醇毒素( DON )對於於益生菌Bacillus coagulans及 Bacillus subtilis之影響。菌體培養實驗於不同時間觀察其成長階段細胞生長情形，並觀察菌體於不同細胞數目與不同階段對毒素吸附之效果。對於這些條件下的變化進行系統性之觀察，而毒素對於凝結芽孢桿菌型乳酸菌具有有菌數提升之效果，但對枯草芽孢桿菌型乳酸菌則無顯著影響。 菌體對於毒素之吸附，吸附率隨著菌數量提升而提升，但菌數之比吸附率則相對減少。可能原因為，隨時間菌數提升，伴隨部分菌體之老化。老化細胞之吸附能力不及年輕細胞。整體而言，菌體吸附效果為 Bacillus coagulans 52至62 %、Bacillus subtilis 47至67 %。以本研究所探討之菌株，做為飼料添加益生菌，除了提供抑制害菌、提昇養分利用率之功能外，同時可降低黴菌毒素之風險，提供動物多一層健康保障。

關鍵字

黴菌毒素 ； 益生菌 ； 吸附劑

並列摘要

The goal of this research is to study the effect of the mycotoxin, Deoxynivalenol (DON), on the probiotic bacteria strains Bacillus coagulans and Bacillus subtilis. The effect of DON on the growth of the two strains, and the adsorption of DON by the cells were investigated. It was observed that the presence of DON stimulated the growth of B. coagulans. DON had no significant effect on the growth of B. subtilis. The adsorption of DON was found to increase during the growth of the two bacteria, but the specific adsorption rate was not proportional to cell numbers. This might be attributed to the possibility that the adsorption capability of the old cell was weaker than the young cells. Overall speaking, the adsorption capability was 52 to 62% for B. coagulans, and 47 to 67% for B. subtilis. The two probiotic strain, if used as additive for animal feed, could provide benefit of lowering the risk of mycotoxin poisoning.

並列關鍵字

Mycotoxin ； Binder ； Probiotics

參考文獻

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