蛹蟲草生產蟲草素最適化培養基之初步探討

Preliminary Study on the Medium Optimization for the Production of Cordycepin from Cordyceps militaris

蔡宇舟(Yu-Jhou Tsai)

指導教授 ： 劉炳嵐

朝陽科技大學/理工學院/應用化學系/碩士(2010年)

摘要

蟲草素(Cordycepin)為蟲草屬中特有的具有生物活性之化合物，而且在蟲草屬中，蛹蟲草所含的蟲草素遠高於其餘蟲草屬的菌種中核?酸成分。本研究利用蛹蟲草(Codyceps militaris)菌株進行生產蟲草素最適化培養基之探討。實驗以選定之碳、氮源加上三種胺基酸(Cysteine、Serine、Glycine)以及在結構上類似蟲草素的腺?(核?酸)，分別試驗其對蟲草素產量之影響。結果發現，三種胺基酸與腺?(濃度皆為20 mM)之添加均對生產蟲草素有增加的趨勢，各實驗組數據顯示與控制組相較，其中Cysteine提升產量最為顯著達4.9倍(147.5mg/L)。若將三種胺基酸(濃度各為10 mM)同時加入培養基中可讓蟲草素產量提升約11倍達到346 mg/L。利用回應曲面法RSM (Response Surface Methology)進行生產蟲草素最適化培養基之研究，實驗結果經數值分析，得到最適化組成為：Glucose 29.73 (g/L)、Purple rice 30.69 g/L、(NH4)2SO4 6.99 (mM)、Peptone 7.56 (g/L)、Cysteine 10.38 (mM)，其蟲草素之理論產量為481.83 (mg/L)。固態培養的部份，以初期不照光，轉色後控制光照時間(光照16 hr以上暗培養8 hr以上)，全程以20

關鍵字

回應曲面法 ； 固態發酵 ； 蟲草素 ； 蛹蟲草

並列摘要

The Cordyceps genus is a name given to themedicinalmushroomthat parasitizes in insects and changes itself to fruiting bodies, and then comes out of the ground. One of the most important species in Cordyceps genus is Cordyceps militaris. The cordycepin biosynthesized in C. militaris is a unique nucleoside analogue, which has a broad spectrum of biological activity, especially as an anti-cancer drug. In this study, the optimizations of culture medium for the production of cordycepin from C. militaris were investigated. In addition to main carbon and nitrogen sources, three amino acids (cysteine, serine, glycine) and a nucleotide structural analogue of cordycepin (adenosine) were studied for their effects on the cordycepin production. The results suggested that all the additives exhibited a positive effect on the cordycepin yields. Among, cysteine had most significant effect on the cordycepin yields, up to 4.9 times (147.5mg/L) higher that of control was obtained. An approximately 11 times higher cordycepin yield was observed (346 mg/L) while three amino acids were added to the medium together. According to the response surface analysis using a central composite design for the production of cordycepin, the predicted optimal composition of medium were: glucose 29.73 (g/L), purple rice 30.69 g/L, (NH4)2SO4 6.99 (mM)、peptone 7.56 (g/L)、cysteine 10.38 (mM), and the yield was estimated to be 481.83 mg/L. For the solid-state cultivation, no light was nessary in the early stage. Photoperiod control (light 16 hr dark for more than 8 hr) was commenced after C. militaris color changed. The culture temperature was maintained at 20

並列關鍵字

Cordyceps militaris ； Cordycepin ； Solid-state fermentation ； Response surface methodology

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