魚針草抗A型流感病毒的研究

Effects of Anisomeles Indica against Influenza A Virus

林詠鈞(Yung-Chun Lin)

指導教授 ： 鄭如茜

朝陽科技大學/理工學院/應用化學系生化科技碩博士班/碩士(2010年)

摘要

流行性感冒病毒 (Influenza virus) 具有高度的致死率並在世界各地造成疾病產生，因此在醫學領域上屬於重點研究的病原體。雖然目前已經發展出兩種抗流感病毒的藥物，但是在最近的研究中指出，發現了某些病毒株已經對這兩種抗流感病毒藥物產生了抗藥性。魚針草 (Ansiomeles indica) 為一年或越年生草本植物，具有抗發炎以及抗 HIV 病毒的活性。為了探討魚針草是否能抗流感病毒，先使用病毒斑試驗做初步的探討。在流感病毒和魚針草共同培養的模式中，隨著魚針草的濃度增加，病毒斑的數量亦隨之減少，另外再檢測受感染的細胞內、外的病毒 RNA 含量，同樣的也隨著魚針草濃度的增加而減少。進而利用幾種不同的試驗，探討魚針草抑制流感病毒感染能力的作用機制。結果顯示，魚針草並不能抑制流感病毒的血球凝集素蛋白和 RNA 聚合?蛋白複合物的活性。然而，魚針草似乎能夠抑制神經胺酸?蛋白的活性。總結以上的實驗結果，魚針草植物具有抗流感病毒的活性，降低病毒的感染。

關鍵字

神經胺酸?蛋白 ； 流感病毒 ； RNA 聚合?蛋白複合物 ； 血球凝集素蛋白 ； 魚針草

並列摘要

Influenza virus is a medically important viral pathogen causing significant mortality, morbidity throughout the world. Although two kinds of anti-viral drugs have been developed, recent studies indicated that drug-resistant influenza viruses have been appeared. Ansiomeles indica O.Kuntze (Labiatae) is an annual and biennial herb. It has been reported that Ansiomeles indica with an anti-inflammation and anti-HIV activities. To understand the anti-influenza virus activity of Ansiomeles indica, plaque forming assay were performed. During viral adsorption, plaque formation was reduced by Ansiomeles indica along with decreased intra- and extracellular viral RNA. To address the molecular mechanisms for Ansiomeles indica in the inhibition of influenza virus infectivity, several assays were performed. Our data demonstrate that Ansiomeles indica did not inhibit influenza haemagglutinin, RNP activities. However, the neuraminidase activity seemed to be inhibited by Ansiomeles indica. Taken together, Ansiomeles indica is a potent herb with anti-influenza viral activity.

並列關鍵字

RNP ； haemagglutinin ； Ansiomeles indica ； Influenza virus ； neuraminidase

參考文獻

[1] M. Burnet and D. O. White, Natural history of infectious disease, 4th ed. Cambridge: University Press, 1972.

[2] T. Thjotta, "Studies on Bacterial Nutrition : I. Growth of Bacillus Influenzae in Hemoglobin-Free Media," J Exp Med, vol. 33, pp. 763-71, May 31 1921.

[3] C. H. A. Wilson Smith, P.PLaidlaw,Morage C.Timbury, "A Virus Obtained from Influenza Patients," Lancet, vol. 1, pp. 66-68, 1933.

[4] T. Francis, Jr., "A New Type of Virus from Epidemic Influenza," Science, vol. 92, pp. 405-408, Nov 1 1940.

[5] R. A. M. Fouchier, V. Munster, A. Wallensten, T. M. Bestebroer, S. Herfst, D. Smith, G. F. Rimmelzwaan, B. Olsen, and A. D. M. E. Osterhaus, "Characterization of a novel influenza a virus hemagglutinin subtype (H16) obtained from black-headed gulls," Journal of Virology, vol. 79, pp. 2814-2822, Mar 2005.