日本麴菌液態醱酵生產幾丁聚醣

Production of Chitosan by Submerged Culture of Asperjillus japonicus

施怡秀(Yi-xiu Shih)

指導教授 ： 許垤棊

大同大學/工程學院/生物工程學系所/碩士(2007年)

摘要

利用日本麴菌的液態醱酵生產幾丁聚醣。當使用葡萄糖作為碳源，添加5 ppm鋅離子不僅能夠促進細胞生長，細胞乾重達每公升8.2公克，亦能增加幾丁聚醣的分子量達到65 kDa以及去乙醯化程度達到96.79％。添加10 ppm鐵離子可獲得幾丁聚醣最大產量每公升43.6毫克，以及最大乾燥菌體的幾丁聚醣含量0.79％。改以蔗糖當作碳源時，添加10 ppm的鐵離子可獲得最高細胞乾重，每公升6.4公克。培養基添加5 ppm錳離子，可獲得幾丁聚醣最大產量每公升22.4毫克，以及最大幾丁聚醣含量占乾燥菌體的0.46％。添加5 ppm鋅離子或10 ppm鐵離子可增加幾丁聚醣的分子量。鐵離子可以提高幾丁聚醣去乙醯化程度。培養基中添加5 ppm鋅離子或錳離子可以促進β-呋喃果糖苷酶的活性，其中，添加5 ppm錳離子可獲得最大的酵素活性。

關鍵字

日本麴菌 ； 幾丁聚醣 ； β-呋喃果糖苷酶

並列摘要

The production of chitosan was conducted by a submerged culture of Asperjillus japonicus. During the fermentation using glucose as carbon source, adding 5 ppm of zinc ion resulted in cell growth up to 8.2 g/L, the chitosan with a molecular weight of 65 kDa and the degree of deacetylation of 96.79%. The maximum yield of chitosan at 43.6 mg/L and the chitosan content of 0.79% were achieved upon ferrous ion at 10 ppm. When sucrose was used as carbon source, the highest production of biomass at 6.4 g/L was obtained upon ferrous ion at 10 ppm. Adding 5 ppm of manganese ion resulted in chitosan up to 22.4 mg/L and a chitosan content of 0.46%. Adding 5 ppm of zinc ion or 10 ppm of ferrous ion gave the molecular weight of chitosan up to 49 kDa. The degree of deacetylation of chitosan was increased by adding ferrous ion. The activity of β-fructofuranosidase was enhanced by adding 5 ppm of zinc or manganese ion in the culture medium. The maximum activity was obtained upon manganese ion at 5 ppm.

並列關鍵字

Asperjillus japonicus ； chitosan ； β-fructofuranosidase

參考文獻

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