- 學位論文
利用左旋C棕櫚酸脂微脂粒包覆熊果素進行經皮輸送
Transdermal Delivery of Arbutin Encapsulated by Ascorbyl Palmitate Liposomes
摘要
本實驗在探討利用非磷脂類脂質左旋C棕櫚酸脂製作微脂粒包覆熊 果素(arbutin)，藉由調控不同變因控制，例如濃度差、不同脂類、不同 界面活性劑等等，進行in vitro 藥物經皮輸送釋放效能，以及進行穿透係 數計算及統計分析、豬皮藥物殘留量測定、螢光測定界面活性劑對微脂 粒之影響等實驗之研究。 由實驗結果得知，包覆5%熊果素之微脂粒，其經皮輸送釋放速率 為19.60nmol/cm2/h；未經由微脂粒包覆之5%熊果素溶液經皮輸送釋放 速率14.79nmol/cm2/h，顯示微脂粒包覆對於親水性藥物具有促進輸送的 作用；在不同包覆濃度變因之下，藥物釋放速率隨濃度上升而增加，以 濃度5%的配方為佳，達到19.60nmol/cm2/h；不同界面活性劑變因之下， 以DA(deoxycholic acid)配方為最佳配方，釋放效能達到 26.32 nmol/cm2/h；不同脂類包覆變因之下，顯示本實驗中脂質不是影響 經皮釋放效能的變因。由穿透係數統計分析得知，熊果素經由包覆後輸 送速率顯著提高。 結果顯示，左旋C 棕櫚酸脂微脂粒在經皮藥物輸送的效能上具有促 進作用，所以利用左旋C 棕櫚酸脂製作微脂粒包覆熊果素是可行的方 法，在未來的發展中，可以利用左旋C 棕櫚酸脂包覆其他藥物來進行微 脂粒製作，同時對其藥物控制釋放上的行為模式進行實驗。
並列摘要
The aim for the research is to encapsulate arbutin by liposome based on non-phospholipids “ascorbyl palmitate”, and then perform transdermal delivery experiments by adjusting different factors, such as different drug concentrations, encapsulating lipids, surfactants etc. Furthermore, we got the physical data, such as permeability coefficients, skin-drug accumulation, and so on. From the result, we observed that the releasing rate of 5% arbutin liposome and 5% non-encapsulated arbutin solution are 19.60nmol/cm2/h and 14.79nmol/cm2/h respectively. It means the encapsulation of arbutin has enhancing effects for transdermal delivery. By adjusting different drug concentration, we observed that releasing rate rises with concentration under saturation concentration range. The best formulation is 5% arbutin and it comes to the number of 19.60nmol/cm2/h. By adjusting different surfactants, DA is the best formulation and it comes to 26.32 nmol/cm2/h. By adjusting different lipids, it showed that the lipid did not affect the release. In conclusion, the non-phospholipids “ascorbyl palmitate” has effects in improving the transdermal delivery efficiency and we could also apply it to other drugs to investigate the behaviors for delivery in the future.