NIPAAm/GMA共聚合凝膠之製備及其性質與應用之研究

STUDIES ON PREPARATION AND PROPERTIES AND APPLICATION OF THE N-ISOPROPYLAMIDE AND GLYCIDY METHACRYLATE COPOLYMERIC HYDROGEL

黃啟泰(Chi-Tai Huang)

指導教授 ： 李文福

大同大學/工程學院/化學工程學系所/碩士(2007年)

摘要

摘要
Part 1
本實驗主要是製備兩種不同系列的溫感性水膠 poly(NIPAAm-co-GMA) 和 poly(NIPAAm-co-GMA-co-DMA), 研究主要以改變單體 NIPAAm/GMA或GMA/DMA的組成測比例來測量膠體的膨潤行為、機械性質的影響。結果顯示,隨著GMA含量的增加,膠體的膨潤度下降。而膠體的機械強度、交聯密度隨著GMA含量增加上升。
在藥物釋放方面,此高分子膠體 poly(NIPAAm-co-GMA) 和poly(NIPAAm-co-GMA-co-DMA) 對非親水性藥物 sulfanilamide ,吸藥量隨著GMA含量增加而增加,而釋放量受到膠體在去離子水中的膨潤度影響

摘要
Part 2
以NIPAAm、GMA 及AA利用交聯劑 N,N-methylenebisacrylamide (NMBA) 製備ㄧ系列溫度酸鹼感應性水膠, 研究主要以改變單體 GMA和AA的組成測比例來測量膠體膨潤度、機械性質的影響。結果顯示,隨著AA含量的增加,膠體的膨潤度上升。而膠體的機械強度隨著AA含量增加降低。此系列膠體展現好的溫度及酸鹼敏感度。
在藥物釋放方面,此高分子膠體 poly(NIPAAm-co-GMA-co-AA) 對非親水性藥物 sulfanilamide ,吸藥量隨著GMA含量增加而增加,而釋放量受到膠體在緩衝液中的膨潤度影響

摘要
Part 3
本研究是以NIPAAm、GMA和DMA或AA製備三種系列溫度敏感性水膠,其中以改變單體中GMA和DMA 或 GMA和AA 的含量來測量對膠體澎潤渡和物理性質的影響。結果顯示,膨潤度隨NIPAAm, DMA 和 AA的含量越多而越高。而膠體的機械強度、交聯密度會隨GMA含量的增加而上升。
在酵素固定化的方面,此三種膠體對固定化胰蛋白具有良好的效果, 此固定了胰蛋白酵素的溫感性膠體可作為親和分離之載體，並直接從蕃薯萃取液中，分離出胰蛋白酵素的抑制劑sporamin,在親合層析後,具有治54%, 53 ﹪和 35% 的sporamin活性被吸附於固定化酵素之膠體,而此胰蛋白脢抑制劑的分子量約20KDa,其分子量大小可藉由SDS-PAGE觀察到

關鍵字

共聚合 ； 凝膠

並列摘要

Abstract
Part 1
Studies on Preparation and Properties of poly(NIPAAm-co-GMA) and poly(NIPAAm-co-GMA-co-DMA) Copolymeric Hydrogels
In this study we synthesized two series of reversible thermo-responsive hydrogel, poly (NIPAAm-co-GMA) and poly(NIPAAm-co-GMA-co-DMA) , The effect of the different monomer ratios of the poly(NIPAAm/GMA) and poly(GMA/DMA) copolymeric hydrogel on the swelling behaviors and physical properties was investigated. The result showed that the swelling ratio in deionized water increased with an decrease of content of GMA in the copolymeric hydrogel. The compressive modulus、crosslink denity and mechanical strength increased with GMA content in the copolymeric hydrogel.
The influence of the copolymeric composition on the drug release behavior in the poly(NIPAAm-GMA ) and poly(NIPAAm-GMA-DMA) hydrogel which were investigated in this study. The loading amount of sulfanilamide increase with an increase of content of GMA in the gel,but the release ratio was affected by the swelling ratio of the gel in the deionized water .

Abstract
Part 2
A series of pH/thermo-sensitivity hydrogels, were prepared from N-isopropylacrylamide (NIPAAm) , acrylic acid (AA), and glycidyl methacrylate (GMA), crosslinked with N,N-methylenebisacrylamide (NMBA) . The effect of the different monomer ratios of GMA and AA in the copolymeric hydrogel on the swelling behaviors and physical properties were investigated. The result showed that the swelling ratio in deionized water and in phosphate buffer solution increased with an increase of content of AA in the copolymeric hydrogel. The compressive modulus,crosslinking density and mechanical strength decreased with an increase of AA content in the copolymeric hydrogel.
The influence of the copolymeric composition on the drug release behavior in the poly(NIPAAm-co-GMA-co-AA ) hydrogel was also investigated in this study. The loading amount of sulfanilamide increase with an increase of content of GMA in the gel,but the release ratio was affected by the swelling ratio of the gel in the deionized water

Abstract
Part 3
Three series of thermo-responsive hydrogel, was synthesized from N-isopropylacrylamide (NIPAAm) , glycidyl methacrylate (GMA), and N,N-dimethylacryamide (DMA) or acrylic acid (AA) . The effect of the different monomer ratios of GMA and DMA or GMA and AA in the copolymeric hydrogel on the swelling behaviors and physical properties were investigated. The result showed that the swelling ratio in deionized water increased with an increase of content of NIPAAm 、DMA and AA in the copolymeric hydrogel. The compressive modulus,crosslink density and mechanical strength increased withGMA content in the copolymeric hydrogel.
The influence of the copolymeric composition on the Immobilized enzyme in the poly(NIPAAm-co-GMA), poly(NIPAAm-co-GMA-co-DMA), and poly(NIPAAm-co-GMA-co-AA) copolymeric hydrogels which was used as a support to immobilize trypsin was investigated. The result showed that the copolymeric hydrogel showed a good immobilized capacity of trypsin. The immobilized trypsin as a ligand of affinity chromatography had 54 , 53, and 35 percent activity of trypsin inhibitor in the crude extract of sweet potato can be recovered by affinity chromatography. The molecular weights of the purified trypsin inhibitors are more than 20 KDa by SDS-PAGE.

並列關鍵字

copolymer ； hydrogel

參考文獻

13 K.S. Jaw, Kow-Jen D, Ming-Tse L., Purification of trypsin inhibitor from sweet potato by applying immobilized trypsin on glutaraldehyde activated chitosan beads, J.The Chinese Institute of Chem Eng 2006 37 (2): 125-129

2. Bae, Y. H.; Okano, T.; Kim, S. W. J Polym Sci Polym Phys 1990, 28, 923.

8. Dong, L. C.; Hoffman, A. S. J Control Release 1986, 4, 223.

13. Kwon, I. C.; Bae, Y. H.; Okano, T.; Bemer, B.; Kim, S. W. Makromol Chem Macromol Symp 1990, 33, 265.

22. Ishihara, K.; Hamad, N,; Kato, S.; Shinihara, I. J Polym Sci Polym Chem Ed 1984, 22, 881.