清酒粕經乳酸菌醱酵產物對抑制黑色素生成之探討

Inhibition of melanin synthesis by sake lees fermented with lactic acid bacteria.

王景平(Jing-Ping Wang)

指導教授 ： 顏聰榮

大同大學/工程學院/生物工程學系所/碩士(2008年)

摘要

黑色素生成作用對於人類皮膚以及毛髮顏色來說是合成色素的重要反應。在人體皮膚上黑色素除了形成顏色之外也是抵擋紫外光及結合自由基的重要物質，酪胺酸酶 (Tyrosinase) 為含有二價銅離子的關鍵黑色素生成酵素。清酒酒粕(Sake lees) 為製造清酒的副產物，內含許多的營養成分。根據文獻指出，清酒酒粕對於製酒者的皮膚具有保濕及美白的功能。
在這篇研究中，利用Lactobacillus GG這株標準菌作為對照組以及四株由本實驗室從本土醱酵產品所篩選出來的植物性乳酸菌藉由添加不同濃度的清酒酒粕 (0 %, 10 %, 20 %, 30 % and 40 %) 去醱酵培養，取已過濾的醱酵上清液去測試對於mushroom tyrosinase活性的抑制效果以及減少小鼠黑色素瘤細胞 (B16-F0 cell line) 黑色素的生成影響。結果顯示，經由Lb. casei E7及E15這兩株乳酸菌與酒粕所醱酵的上清液分別具有60 %及40 %抑制mushroom tyrosinase的活性；也能分別具有42 %及26 %抑制黑色素生成的效果。將具有抑制成分的上清液經由HPLC以及Micro FT/IR這兩台儀器去確認成分後，結果顯示，抑制成分可能是亞麻油酸 (Linoleic acid)。
根據這篇研究指出植物性乳酸菌藉由添加清酒酒粕醱酵培養後能產生具有美白效果的亞麻油酸，而乳酸為乳酸菌醱酵作用的主要產物，也是人體皮膚中天然保濕因子 (NMF) 的成分之一，因此，具有能發展成為美白化妝品的潛力。

關鍵字

清酒粕 ； 乳酸菌 ； 黑色素

並列摘要

The melanin-synthesizing is an important role of pigmentation on human skin and hair color expression. It is also an important defense of human skin against UV-induced injury and to scavenge oxidative free radicals. Tyrosinase is a copper-containing enzyme involved in melanin synthesis. Sake lees, byproduct in the production of sake, are rich in nutrition. It is reported that the sake lees are good for sake brewers to keep their skin moisture and whitening.
In this study, Lactobacillus GG and the four lactobacillus strains isolated from local Taiwanese fermented vegetables were used to fermented with different concentrations of sake lees extract (0 %, 10 %, 20 %, 30 % and 40 %), and the supernatant filtrates were used to test mushroom tyrosinase inhibition activity and decrease melanin production of B16-F0 cell line. According to the results, two supernatant filtrates from Lb. casei E7 and E15, could obviously inhibit the mushroom tyrosinase activity of 60 % and 40 %, and decrease melanin contents of B16-F0 cell line of 42 % and 26 %, respectively. The tyrosinase inhibitory compounds contained in supernatant filtrates were subjected to HPLC and Micro FT/IR to identify the compounds as linoleic acid.
Furthermore, lactic acid, the major product of lactic acid bacteria fermentation, which is a main component of human natural moisturizing factor (NMF). Therefore, the fermentation products from sake lees by the two lactobacilli will be very useful for application in the cosmetic field.

並列關鍵字

Sake lees ； Melanin ； Lactic acid bacteria

參考文獻

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