摘要
隨著組織工程研究的蓬勃發展，對於幫助組織或器官之修復被視為重要課題之一，而新的組織生長與發育需要適當的血管用以傳送養分以及移除廢物。加上有研究指出腫瘤的生長也與血管新生有相關性，也就是說血管新生能夠促進腫瘤增殖與轉移。如果我們能夠利用電腦來模擬並預測血管新生的現象，期望對於未來在治療腫瘤與組織修復，能夠給予相當程度上的幫助。 本篇研究利用human umbilical vein endothelial cells（HUVEC）來進行in vitro的血管新生實驗，藉由調控不同濃度的血清比例（0％、0.5％、2％、10％）並添加100 ng/ml的vascular endothelial growth factor（VEGF）來誘導HUVEC形成類微血管狀結構。結果發現，隨著血清濃度的提升形成類微血管結構的完整度也越高，推論HUVEC形成類微血管結構與血清的多寡有正相關性。電腦模擬則是利用已知的數學模型轉化成Matlab的程式語言，並藉由幅射型基底函數帶入實驗之參變數來進行模擬，以探討藉由程式模擬的可行性與準確性。
並列摘要
Blood vessels are needed for nutrition transporting and metabolic waste removal in the growth of new tissues. Many reports indicated that tumor’s growth had connection with angiogenesis. Angiogenesis can assist proliferation and transfer of tumor cells. If we can use computer as a tool to simulate and forecast angiogenesis, the result may has a lot of help for tumor cure and tissue repair. In this study, we use human umbilical vein endothelial cells (HUVEC) to do in vitro angiogenesis experiment. We regulated the concentration of serum ( 0%, 0.5%, 2% and 10% ) and added 100 ng/ml vascular endothelial growth factor to induce HUVEC capillary-like structure formation. In result, we discovered that the completion of capillary-like structure increased as the concentration of serum did. Therefore, capillary-like structure and concentration of serum can be correlated. In the process, we transform angiogenesis mathematical model into program of MATLAB. A substitution of experiment parameters into a radial basis function for simulation was followed. Depend on simulation output, we can appraise accuracy and feasibility of simulation.