- 學位論文
利用電漿處理及UV光接枝聚合固定聚丙烯酸藥物釋放水膠並固定幾丁聚醣/褐藻酸於316L不鏽鋼表面
Immobilization of poly(acrylic acid) by using plasma treatment and UV-induced polymerization then immobilization Chitosan / Alginate mixed solution on the 316L stainless steel.
摘要
不鏽鋼的金屬性質很難表面固定高分子，因不鏽鋼本身為無機基材，故本研究利用電漿聚合法先沉積一層界面層，然後以表面自由基光接枝聚合法成長聚丙烯酸水膠，即可利用此酸鹼值感應型水膠進行藥物釋放，並且能夠在其表面再沉積一層HMDSZ( Hexamethyldisila- zane )薄膜作為藥物釋放之阻隔層，以延緩及持續藥物釋放的時間，之後能利用浸泡EDC / NHS方法定幾丁聚醣/褐藻酸於表面，形成適合細胞貼附之表面。 而經過電漿後處理及表面接枝聚合，可改變成親水性，有利於幾丁聚醣的固定。結果為利用電漿沉積後接枝聚丙烯酸之固定量(2.08 mg/cm2)比只有電漿後處理的固定量(0.24 mg/cm2)多；另外隨著幾丁聚醣/褐藻酸混和比對固定量的影響，發現褐藻酸比例增加時，固定量有增加趨勢，固定量增加，表示褐藻酸比例上升，能有利於EDC / NHS方法固定在表面帶陰電性高分子上。 結果發現利用HMDSZ電漿沉積膜，能夠延緩聚丙烯酸的膨潤率，從約43～47倍，降低至25～30倍，但在藥物釋放的情況下並不明顯，只有初期有延緩作用。
並列摘要
The stainless steel is one kinds of an inorganic material, it`s difficult to immobile the polymer due to its metallic properties. In this study, using plasma treatment to deposit an interface layer, then using UV-induced polymerization to form poly(acrylic acid) hydrogel. It could be used in drug delivery system which is one kinds of pH sensitive hydrogel, and it could be deposited a layer of HMDSZ film to stem drug released. Then immerse into EDC/NHS solution to immobile chitosan and alginate on the surface which suitable for cell adhesion. The surface wettability will become hydrophile after UV-induced polymerization.It is contributive of chitosan immobilization. Then the amount of chitosan by using acrylic acid immobilization is about 2.08mg/cm2, it is much higher than which is only by surface activation production about 0.24mg/cm2. Besides, the effect of amount of chitosan/alginate mixed solution was followed by alginate ratio increase. As the result of increasing the alginate ratio will favorable for surface immobilization. The results indicate that plasma deposited HMDSZ film can suspend swelling ratio of poly(acrylic acid) from 43~47 to 25~30 ratio, but in the drug release system is not significantly.