以分子模版進行乙酸苯乙醇及乙酸苯乙酯選擇性萃取之研究

Selective Extraction 2-phenylethyl alcohol and 2-phenylethyl acetate by Molecularly Imprinted Solid Phase Extraction

盧毓祥(Yu- Shiang Lu)

指導教授 ： 張志雄

大同大學/工程學院/化學工程學系所/碩士(2010年)

摘要

本研究主要目標是製備具專一吸附乙酸苯乙醇及乙酸苯乙酯的分子模板，以期能在生產此二成份之發酵槽內現場移除此二成份，降低P. fermentans L-5菌體受到此二成份毒化之效應。經過各項變因的實驗結果歸納出製備乙酸苯乙醇分子模版時，以交聯劑(PEG400DMA)與功能性單體(1-VI)之比為3:2，功能性單體(1-VI)與目標分子(2-PE)之比為3:1及溶劑（Toluene）與單體（1-VI）比（mL:g）為4:1，乙酸苯乙醇分子模版對目標分子會有最大吸附量41.25 mg/g及最高選擇率2.40。而在製備乙酸苯乙酯分子模版時，以交聯劑(NMBA)與功能性單體(HEMA)之比為3:2，功能性單體(HEMA)與目標分子(2-PEAc)之比為4:1及溶劑（Dimethyl sulfoxide）與單體（HEMA）比為（mL:g）為8:1，製備之乙酸苯乙酯分子模版對目標分子會有最大吸附量58.33 mg/g及最高選擇率2.18。與常用來吸附此二成份之市售DIAION®HP20來比較，實驗結果可知分別加入所製備之兩種分子模版吸附並脫附出乙酸苯乙醇與乙酸苯乙酯的總量會高於DIAION®HP20的表現。

關鍵字

乙酸苯乙酯 ； 乙酸苯乙醇 ； 分子模版

並列摘要

In this work, we were aimed to synthesize the molecularly imprinted polymers (MIP) that can remove specifically 2-phenylethyl alcohol and 2-phenylethyl acetate from a fermenter in order to reduce the poison effect of these two components on the P. fermentans L-5 cells adopted for fermentation. From the experiments on the possible control variables, we find that for synthesizing the MIP that can remove specifically 2-phenylethyl alcohol, the optimizing recipe and operating conditions are (a) the mole ratio of cross-linking (PEG400DMA) to the functional monomer (1-VI) 3:2, the molar ratio of functional monomer to the template 3:1 and the ratio of solvent (Toluene) to the functional monomer (1-VI) is 4:1 (mL/g). The adsorption capacity of the obtained MIP for 2-phenylethyl alcohol is 41.25mg/g while the selectivity of 2-phenylethyl alcohol to 2-phenylethyl acetate is 2.4. We also find that for synthesizing the MIP that can remove specifically 2-phenylethyl acetate, the optimizing recipe and operating conditions are (a) the mole ratio of cross-linking (NMBA) to the functional monomer (HEMA) 3:2, the molar ratio of functional monomer to the template 4:1 and the ratio of solvent (Dimethyl sulfoxide) to the functional monomer (HEMA) is 8:1 (mL/g). The adsorption capacity of the obtained MIP for 2-phenylethyl acetate is 58.33mg/g while the selectivity of 2-phenylethyl alcohol to 2-phenylethyl acetate is 2.18. In comparison with the molecular sieve DIAION®HP20, the experiments demonstrated that the prepared MIPs for these two components are superior to DIAION®HP20 in adsorbing and desorbing these two components from the simulated solution of the fermenter.

並列關鍵字

2-phenylethyl acetate ； 2-phenylethyl alcohol ； Molecularly imprinted polymer

參考文獻

【3】L. Janssens, H.L. De-Poorter, L. Demey, E.J. Vandamme, N.M. Schamp “Production of flavours by microorganisms”, Process Biochem, 1992, 27, 195-215.

【6】李建育 “以固定化細胞Pichia fermentans L-5生產乙酸苯乙醇及乙酸苯乙酯之研究”, 碩士論文, 大同大學化學工程學研究所, 民國96年.

【7】林子揚 “在發酵槽中以高密度細胞Pichia fermentans L-5生產乙酸苯乙醇及乙酸苯乙酯之研究”, 碩士論文, 大同大學化學工程學研究所, 民國93年.

【9】F.H. Deickey “Proceeding of the National Academy Science of the United State of America” 1949, 25, 227.

【12】B. Sellergren, B. Ekberg , K. Mosbach “Molecular Imprinting of Amino Acid Derivatives in Macroporous Polymers” Journal of Chromatography 1985, 347, 1-10.