以石英晶體微天平為基礎之細胞培養平台系統

CELL-CULTIVATION BASED ON QUARTZ CRYSTAL MICROBALANCE

李昭法(Chao-Fa Lee)

指導教授 ： 顏聰榮

大同大學/工程學院/生物工程學系所/博士(2011年)

摘要

石英晶體微天平(Quartz Crystal Microbalance, QCM)作為微量物質量測的工具，通常視為質量感測平台。其原理在石英晶體上施加壓力，導致石英晶體的物理形變，此為壓電效應，藉由石英晶體表面的附著質量與石英振盪頻率的變化關系，可推算出石英晶體表面質量的微小變化，藉此石英晶體微天平廣泛應用於精密的量測。另外由於石英晶體的感測方式僅涉及表面交互作用，特別是在液態環境中的量測，在QCM感測發生許很多讓人感受興奮的結果。
在液態環境中，石英晶體不僅受到外在物理形變，包括溶液離子性及黏稠性，均會反應在石英晶體壓電效應，頻率變化只是綜合反應其中的一項。假設介質的特性是由電感、電容、電阻之電器元件所組成，各項參數均會影響石英晶體電器特性而改變其壓電效應。許多實驗結果顯示，石英晶體微天平除重量變化外亦可以用來量測細胞表面黏著性、細胞生長變化以及細胞受外部刺激時反應。由於這樣的應用也讓石英晶體微天平的開發有著更多樣性方向。
本實驗開發 QCM 細胞培養技術，為多功能整合性石英晶體微天平。 利用比較器產生石英晶體振盪。亦利用持樣放大電路量測石英晶體振盪振幅。並且提供阻抗分析及跨上皮細胞阻抗的量測。藉由這個平台，在受控制環境下，探討QCM及細胞表面作用反應。

關鍵字

阻抗分析 ； 振幅 ； 振盪頻率 ； 石英晶體微天平 ； 生物晶片 ； 跨上皮細胞阻抗

並列摘要

A QCM (Quartz-Crystal Microbalance) examined as the micro-material amount, is usually regarded as a mass detection platform. A micro-material exerts pressure on the quartz crystal causing the physics deformation of the quartz crystal, this is a piezoelectricity effect. The change relation that mass alteration and quartz shift frequency can be calculated by superficially interaction on the quartz crystal. Because the way of detecting by the quartz crystal only involves the surface reciprocation, in addition, that makes experience to have a lot of excited result. Especially micro-materials among liquid environment examine to happen in living beings detect.
In the liquid environment, the QCM not merely response external physics deformation, including the concentration of ion and the adhesion of subject, will react in the quartz crystal piezoelectricity effect. The frequency change is not only one that an interconnected system cross reaction. Suppose the characteristic of the medium which consist of the electric components of the inductor, capacitance, resistance, they can influence QCM electric apparatus and change the piezoelectricity effect and make the results different. A lot of experimental results show, QCM can be used to observe the cell growth condition. Such application make there are more diversified directions in the development of the QCM.
This study develops a QCM Thermostatically bio-cultivation was a combining multi-functional QCM instrument. The quartz crystal resonance was produced by using two comparators and an op-amplifier. We can capture the oscillation frequency, also can capture the amplitude of QCM by using sampling and hold circuit. And provide impedance analysis and the trans-epithelial electric resistance (TEER) measurement. With this platform, we offer the analysis of biotechnology to probe the interaction between the QCM surface and the membrane of cell in the liquid with environment controlled.

並列關鍵字

amplitude ； frequency ； QCM ； biosensor ； impedance analysis ； TEER

參考文獻

辛聖文。2006。QCM於黏彈性生醫介質之量測，臺灣大學碩士論文，台北

葉子源，2006，整合電化學檢測功能的雙模態石英微天平振盪器之開拓與研究， 臺灣大學碩士論文，台北

陳俊光。2007。同時解析分子質量與液相阻尼之相位補償式石英微質量天平，國立台灣大學博士論文，台北

楊坤松。2005。TSM振盪器應用於生化感測之研究，臺灣大學碩士論文，台北

廖紹安。2008。多平台電極石英板最佳化設計之研究，臺灣大學碩士論文，台北