- 學位論文
花青素添加入明膠電紡於藥物載體應用之評估
Evalution of Proanthocyanidin on gelatin fiber of electrospinning for drug carrier
摘要
本研究的目的為使用靜電紡絲之技術製備奈米纖維薄膜，並於材料內添加花青素，並觀察花青素對於材料整體的物化性、生物適應性的影響，以及維他命C磷酸鎂鹽在電紡纖維薄膜中釋放之情況。 實驗中，添加花青素之明膠溶液經靜電紡絲製備成薄膜後，使用戊二醛交聯，藉由掃描式電子顯微鏡 (SEM) 來觀察纖維直徑和型態。接著利用不同的浸泡時間和Ninhydrin assay來證實添加花青素對靜電紡絲的降解及交聯度的影響。藥物釋放情況，則是依靠HPLC分析釋放液中的維他命C磷酸鎂鹽之濃度。藥物的活性，使用DPPH清除自由基的實驗來評估。最後，以L-929纖維母細胞和材料共培養，以評估其生物適應性。 結果顯示，無添加花青素的纖維直徑為 328±33.06 nm，而有添加花青素則為 468±29.11 nm，添加花青素會造成纖維直徑的增加。在經蒸氣交聯的過程中，明膠仍可有效被交聯，並且保護纖維結構避免被破壞，而添加花青素可提高整體交聯度。由藥物釋放曲線圖可得知，維他命C隨著時間增加釋放的濃度，並在一個小時後呈現平穩的釋放。添加花青素後，整體材料的自由基清除率可達65%，可維持藥物活性，並且不會對纖維母細胞產生毒性。
並列摘要
One of aims in this study is to prepare a nano-fiber membrane by using a electrospun technology. Another is to evaluate the effect of Oligomeric proanthocyanidins (OPCs) -addition on characters of nanofiber drug carrier. In this study, electrospun OPCs contained gelatin fibers were characterized using scanning electron microscopy (SEM), Ninhydrin assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging were used to evaluate the antioxidant properties. The release of Magnesium L-ascorbyl- 2-phosphate from the electrospun fibers was determined by HPLC. Cell cultures were evaluated in terms of cell cytotoxicity and morphology. The result from SEM analysis of the blend fibers containing OPCs showed that the average fiber size was increased from 328.6±33.06 nm to 486.1±29.11nm, and the crosslinking index increased from 53.41% to 64.07%. OPCs lead to an alteration of the physicochemical properties of electrospun membrane. Our results indicate that all OPCs -gelatin blend electrospun membrane supported L-929 cell attachment and nontoxic.