以脈衝氧化法表面處理牙科植體用鈦合金之研究

Surface Modification of Dental Implant Titanium Alloy by Pulse Oxidation Method

羅君儀(LOH KUAN YEE)

指導教授 ： 許學全

中臺科技大學/健康科學院/牙體技術暨材料系碩士班/碩士(2019年)

摘要

鈦及鈦合金被廣泛應用於整形外科及牙科植體，是因為具有很好的機械性質及耐蝕性。然而，鈦植體因為應力屏蔽效應，生物惰性及缺乏足夠的抗菌性，將導致植體在口腔內植入後有失敗的風險。一些研究報告指出，透過陽極氧化在植體表面摻入銀粒子可改善植體的抗菌性。因此，本研究擬探討含銀的新型Ti-Nb-Mo（TNM）鈦合金的抗菌性及其生物相容性。實驗將會在室溫下使用脈衝氧化對TNM合金進行表面處理，並透過調整電解液的濃度，成分及脈衝的參數（時間、電壓及工作週期），以製備具有生物相容性及抗菌性的植體。
結果顯示：TNM合金以脈衝氧化製作奈米管，表面製備的奈米管受電壓、時間及工作週期的影響，其中以20 V進行60 min可製作出均勻的奈米管表面。當脈衝頻率1000 Hz、工作週期10%及加入濃度3 mM AgNO3時，獲得奈米管直徑約為50 nm，且表面銀含量最多且分佈最均勻。EDS mapping分析證明銀成功地摻入到TNM-nt的表面。經由400℃進行3 hr熱處理後奈米管形貌、長度並未隨著熱處理而改變，合金表面有銳鈦礦相形成。AFM分析顯示：奈米管TNM合金（TNM-nt）具有較高粗糙度。水接觸角結果顯示：製備奈米管表面所測量角度均小於30度呈現良好的親水性。XPS分析顯示：含銀奈米管TNM合金表面的銀為金屬銀。生物相容性結果顯示：細胞與含銀奈米管表面具有良好的貼附效果。抗菌結果顯示：含銀奈米管之菌落數明顯降低。以上結果顯示：TNM合金經由脈衝陽極氧化法後，成功在TNM合金表面上製備含銀奈米管，也得知含銀奈米管具有良好的抗菌效果及生物相容性。

關鍵字

鈦合金 ； 脈衝陽極氧化 ； 奈米管 ； 銀 ； 抗菌性

並列摘要

Titanium is widely used in orthopedics and dental implants because of its excellent mechanical properties and corrosion resistance. However, the stress shielding effect, biological inert and lack of adequate antibacterial property of titanium lead to the failure of bone integration in the oral cavity. Therefore, this study aimed to enhance the antibacterial activity and biocompatibility by incorporating silver on Ti-Nb-Mo (TNM) alloy. The surface of TNM alloy was modified by pulse anodization at room temperature by controlling the concentration of electrolyte, composition and pulse parameters (e.g., time, voltage, working cycle, etc.). The results showed that the nanotubes on the surface of TNM alloy are affected by voltage, time and duty cycle. EDS mapping analysis confirmed that silver was successfully incorporated on the surface of the TNM alloy. An anatase phase was found on the surface of the alloy after heat treatment at 400°C for 3 hr. AFM analysis showed that the nanotube TNM alloy (TNM-nt) had a higher roughness. The nanotube TNM alloy (TNM-nt) and silver-containing nanotube TNM alloy (TNM-nt-Ag) had a water contact angle of less than 30 degree which indicated good hydrophilicity. XPS analysis showed that the silver on the surface of the silver-containing nanotube TNM alloy is metallic silver, which could achieve the antibacterial effect. Biocompatibility test results showed that the cells had a superior adhesion to the surface of the silver-containing nanotubes. The antibacterial test results showed that the number of colonies on silver-containing nanotubes was significantly reduced. The above results showed that the silver-containing nanotubes on the surface of TNM alloy was successfully prepared by pulse anodization, and had excellent antibacterial effects and biocompatibility.

並列關鍵字

Titanium alloy ； Pulse anodization ； Nanotube ； Silver ； Antibacterial property

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