單細胞光學微操弄應用於臨床研究的可行性

Application feasibility of optical micro-manipulation to single cells for clinical studies.

黃閔輝(Min-hui Huang)

指導教授 ： 林志郎

中臺科技大學/健康科學院/醫學工程暨材料研究所/碩士(2012年)

https://doi.org/10.6822/CTUST.2012.00042

摘要

本研究利用雷射光鉗操弄微米級微粒子之特性，進行單細胞之捕捉與拖曳操弄，探討其應用在臨床研究與檢測的可行性，實驗標的物分別為直徑約20 µm和1 µm的羊水細胞及金黃色葡萄球菌，內容包括：(1)利用光鉗揀選置於微流道晶片之羊水細胞，因應實際流道尺寸與細胞拖曳路徑，探討較佳的光鉗操弄參數，包括雷射功率和拖曳速度，進行細胞分離； (2) 針對金黃色葡萄球菌進行光鉗作用力量測，探討其不同等級抗藥性所獲致的光鉗作用力之關係，試著區分萬古黴素敏感性金黃色葡萄球菌 (VSSA) 菌株及萬古黴素中度抗性金黃色葡萄球菌(VISA)菌株。
以上實驗成果預期將有助於單細胞培養及單細胞全基因體定量分析，以及提供臨床醫師較快速的檢測方法，以便即時正確用藥。

關鍵字

光鉗 ； 羊水細胞 ； 萬古黴素中度抗性金黃色葡萄球菌

並列摘要

In this study, the manipulation characteristics of laser tweezers exerted at micro-sized particles are applied for investigating the feasibilities of clinical studies and examinations of single cells by trapping and dragging the experimental subjects: amniotic fluid cells (20 µm) and staphylococcus (1 µm). The experiments include (1) to collect the amniotic fluid cells which are put in the microfluidic chip for the cells separation: to investigate the practical operation parameters, i.e. laser power and dragging speed, in order to harmony with the real dragging route in the micro-channels; (2) to measure the exerted forces of laser tweezers which are at the different resistances of staphylococcus aurous in order to distinguish the strains of VSSA and VISA.
The above experimental achievements are expected to contribute on the development of the culture and the quantitative analysis of whole genome of single cell, and a fast examination of staphylococcus aurous for medical doctor to prescribe for treatment.

並列關鍵字

VISA ； Optical tweezers ； Amniotic fluid cells

參考文獻

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