三維微結構化蛋白質於微流道晶片之應用

近年來生醫晶片(Bio-chip)的發展帶來許多生醫研究與臨床醫學的突破，並已有商業化產品提供方便的檢驗。其概念乃結合微機電、生物化學和生物技術等，集合於一小玻片上，以便進行各項研究與臨床應用，具有簡便、快速，低耗材和準確的優點。本研究乃利用雙光子聚合微製造技術，於微流道中將特定蛋白質或抗體聚合成三維微結構，探討其應用於生物感測晶片的可行性。研究內容包括：(1)將特定蛋白質或抗體以雙光子聚合製造方法製成平板結構，以金黃色葡萄球菌和紅血球為標的物，以雷射光鉗為操弄工具，探討其結構化(structuration)是否仍具其生物功能(function)，驗證其做為生物感測器的可行性；(2)測試上述生物感測器置於微流道中之生物感測效果，並藉由改變平板斜度提高感測效果。本研究之實驗結果預期將可提供一項製造簡便、穩定和方便觀察的生物感測晶片。

關鍵字

雷射光鉗；生醫晶片；蛋白質結構化；雙光子聚合

並列摘要

Recently the development of bio-chip has been contributing on the breakthrough of biomedical engineering. The “Bio-chip” integrated with the techniques of MEMs, biochemical, and biological technology on a cover glass, is coming to notice since it is convenient, rapid, low-consumables, and accuracy for various biological research and clinical diagnosis. Some commercial products have been used for convenient inspections. In this thesis, a feasibility study is performed by employing the technique of two-photon polymerization (TPP) to fabricate 3-D structured specialized protein or antibody in microfluidic channel, which can be used as the detector of bio-chip. The content includes: (1) to fabricate mechanical structured specialized protein or antibody by TPP as a flat, and then utilize optical tweezers to manipulate the objects, such as Staphylococcus aurous and RBCs, to verify their biological function for the application of bio-detector; (2) to improve the sensing result by changing the slope of flat in the micro-channel. The experimental result is expected to contribute a simply fabricated, stable, and easily observed detector for bio-chip applications.

並列關鍵字

Bio-Chip ； Three-dimensional protein structure ； Two-photon polymerization ； Optical Tweezers

參考文獻

[1] H.-B. Sun, S. Matsuo, and H. Misawa, “Three-dimensional photonic crystal structures achieved with two-photon-absorption photopolymerization of resin,” Applied Physics Letters, Vol. 74, 1999, pp. 786-788

[3] S. Maruo, O. Nakamura, and S. Kawata, “Three-dimensional microfabrication with two-photon-absorbed photopolymerization,” Optics Letters, Vol. 22, No. 2, 1997, pp. 132-134

[6] M. Goepper-Mayer, “Ober Elementarakte mit zwei Quantensprungen,” Annalen der Physik, Vol. 9, No.2, 1931, pp. 273-294.

[9] M. Horiyama, H.-B., Sun, Miwa, M., Matsuo, S., and H. Misawa, “Three-dimensional Microstructures Created by Laser Microfabrication Technology,” Japanese Journal of Applied Physics, Vol.38, No. 2B, 1999., pp. L212-L215.

[11] S. Maruo, O. Nakamura, and S. Kawata, “Three-dimensional microfabrication with two-photon-absorbed photopolymerization,” Optics Letters, Vol.22, No. 2, 1997, pp. 132-134.

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三維微結構化蛋白質於微流道晶片之應用

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