臺灣鋏蠓化學感應蛋白之表現與分佈

Expression and distribution of a chemosensory protein in Forcipomyia taiwana

藍俐惠(Li-Hui Lan)

指導教授 ： 李憲明 ； 林榮芳

中臺科技大學/健康科學院/醫學生物科技研究所/碩士(2011年)

https://doi.org/10.6822/CTUST.2011.00049

摘要

化學感應蛋白（chemosensory proteins, CSPs）為分子量約10 ~ 12 kDa的分泌性蛋白質，構造類似球狀，其內部形成疏水性核心，可以和長鏈脂肪族化合物及芳香族化合物結合。臺灣鋏蠓具有與其他昆蟲類似的CSP，其二級結構主要為α-helices，且與長鏈脂肪族化合物具有親和性。為了分析臺灣鋏蠓CSP在不同發育階段的表現情形，本論文利用北方墨點法及西方墨點法進行測試，分析的結果顯示CSP自幼蟲的階段即有少量表現，到達成蟲時表現最高。而在雌性及雄性成蟲則無太大差異。由結果推測，臺灣鋏蠓CSP的表現可能在轉錄階段調控。

關鍵字

北方墨點法 ； 化學感應蛋白 ； 臺灣鋏蠓 ； 西方墨點法

並列摘要

Chemosensory proteins (CSPs) are secreted proteins of 10-12kDa. The conformation of CSPs is globular with a hydrophobic channel within it. The channel is responsible for binding with ligands as long-chain aliphatic on aromatic compounds. The family of CSPs can be divided into several types according to expression profile and function. There is one CSP characterized in Forcipomyia taiwana by its sequence similarity with that of other insects. This protein has a secondary structure rich in α-helice and can associate with hydrophobic compounds. Through Northern and Western blotting, the characteristics of the expression profile of F. taiwana CSP at different stages were identified. The results revealed that level CSP of F. taiwana was low since larvae stage and reached peck in the adult stage. Moreover, no significant difference of expression levels of CSP was identified between genders. According to the identity of the results of Northern and Western blotting, the expression of CSP from F. taiwana is mainly regulation at the transcriptional stage.

並列關鍵字

Northern blotting ； Western blotting ； Forcipomyia taiwana ； chemosensory proteins

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