分析柵藻分離株以紫外線誘導後生長與油脂累積的變化

The Changes of Growth and Lipid Accumulation of Scenedesmus Isolate induced by UV Light were Analyzed

林安婕(AN-JIE LIN)

指導教授 ： 林靜瑩 ； 李孟娟

中臺科技大學/健康科學院/醫學影像暨放射科學系暨研究所/碩士(2017年)

摘要

在微藻生物柴油 (biodiesel) 的大規模生產中，高生物質產量 (biomass productivity) 和高油脂含量的藻種是關鍵。為了獲得這樣的微藻，本研究利用紫外線輻射誘變本實驗室所分離之微藻 FGA326S (Secnedesmus) 並以二階段方式培養，以尼羅紅 (Nile red) 螢光染色技術與顯微鏡觀察藻體油脂分佈，並同時萃取微藻油脂，分析比較油脂比例（倍數）與油脂質量（百分比），以建立紫外線輻射誘變最適條件，並以此最適條件進行紫外線輻射誘變篩選以獲得 FGA326S 紫外線處理過之分離藻株，最後利用顯微鏡觀察 FGA326S 野生藻株 (wild type, WT) 與紫外線處理過之分離藻株的細胞型態，並同時進行比較 FGA326S 野生藻株與紫外線處理過之分離藻株生長變化與油脂含量分析。實驗結果顯示，以紫外線輻射強度 UV-A+UV-B 照射 30 min，FGA326S 藻體油脂比例（倍數）與油脂質量（百分比）變化最大，以此條件進行 FGA326S 藻種誘變及培養，篩選出 6 株生長情況最好之微藻紫外線處理過之分離藻株，分別命名為 M1~M6，比較野生藻株與紫外線處理過之分離藻株 M1~M6 的油脂比例（倍數），得知 M1 與 M5 具有含油量高的特性，是十分具有生產油脂潛力的突變分離藻株，後續將進行相關特性分析，以開發成為能源微藻的用途。

關鍵字

紫外線 ； 微藻 ； 油脂 ； 尼羅紅 ； 生質能

並列摘要

In the large scale production of microalgae biodiesel, high biomass productivity and high the lipid content of microalgae is the key. In order to obtain such microalgae, the microalgae of FGA326S (Secnedesmus) isolated from our laboratory was induced by ultraviolet radiation and cultured in a two-stage manner, and the lipids distribution of microalgae was observed by Nile red fluorescence staining technique and microscope equipment, and the lipid content was extracted simultaneously, to analyze and compare the lipid ratio (multiples) and the lipid content (percentage), in order to establish the optimum conditions for ultraviolet radiation treatment, and to obtain the FGA326S UV-treated strains to isolate the microalgae strains by ultraviolet radiation treatment, and finally to observe the cell phenotype of FGA326S wild type and UV-treated strains, and the growth changes and the lipid content of FGA326S wild type and UV-treated strains were analyzed. The results of this study showed that the lipid ratio (multiples) and the lipid content (percentage) of FGA326S microalgae change biggest when irradiated with UV-A+UV-B for 30 minutes. Mutagenesis and culture of FGA326S microalgae were carried out under this condition, and 6 strains the best growth of microalgae UV-treated strains, respectively, named M1~M6, comparison of wild type and mutated isolated strains M1~M6 lipid ratio (multiples), that M1 and M5 have the characteristics of high the lipid content, is a UV-treated strain of microalgae that has a great potential for producing lipid, and subsequent analysis of relevant properties to develop its use as an energy microalgae.

並列關鍵字

Ultraviolet ； Microalgae ； Lipid ； Nile red ； Biomass

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