活性污泥生物膜程序分解酚和二氯酚之動力研究

Kinetics of Biodegradation of Phenol and 2-Chlorophenol by Acclimated Activated Sludge in a Biofilm Process

吳佳鴻(CHIA-HUNG WU)

指導教授 ： 林燕輝

中臺科技大學/健康科學院/環境與安全衛生工程系碩士班/碩士(2018年)

摘要

氯酚是在許多人為活動期間發生的異生物污染物，並被釋放到環境中。這些化合物廣泛用作防蛀劑，殺蟎劑，殺菌劑，殺藻劑，殺真菌劑和木材防腐劑以及染料和藥物的合成。由於其具高度毒性，工業廢水中的氯酚污染物造成嚴重的生態問題。由於其對生態危害性，氯酚污染物生物降解成為大家關注的話題和研究的目標。
本研究採用批次試驗，探討微生物降解酚與二氯酚之生物降解動力。活性污泥經由兩次酚和二氯酚初始濃度的添加，使酚和二氯酚分解菌成為優勢菌種。此外，利用批次試驗推求菌種的最大比生長率(μmax)、半飽和常數(Ks)以及抑制常數(Ki)，並且評估微生物對雙重基質的生物降解率。
批次試驗是利用共代謝作用來進行酚與二氯酚生物降解。酚與二氯酚的初始濃度為17.2 mg/L及14.3 mg/L、21.2 mg/L及18.6 mg/L、25.3 mg/L及23.4 mg/L、30.4 mg/L及29.1 mg/L去除至100%的天數分別為8、15、23、25天。連續流試驗結果顯示在穩定狀態下，反應槽操作時間從96.5至100.5小時，酚與二氯酚進流濃度分別為12.66及9.25 mg/L，酚和二氯酚去除率為100%。本研究中實驗結果顯示藉由陶瓷顆粒附著生物膜程序能有效降解廢水中酚和氯酚污染物的濃度。

關鍵字

活性污泥 ； 生物降解 ； 酚 ； 二氯酚 ； 共代謝

並列摘要

Chlorophenols are xenobiotic contaminants that occur during numerous anthropogenic activities and become released into the environment. These compounds are widely used as mothproofing agents, miticides, germicides, algicides, fungicides and wood preservatives as well as for the synthesis of dyes and drugs. Owing to their biocide properties, chlorophenols in industrial wastewater pose serious ecological problems as environmental pollutants. Because of their ecological risk, chlorophenols become the target of investigations focusing on their possible biodegradation.
In this study, we used batch tests to investigate the kinetics of phenol and 2-chlorophenol biodegradation. The phenol and 2-chlorophenol degrading cultures became the dominant cultures due to the incubation of activated sludge by addition of initial phenol and 2-chlorophenol twice. Moreover, we used batch tests to determine the maximum specific growth rate (μm), half-saturation constant (Ks) and inhibition constant (Ki) and evaluate the dual-substrate biodegration by cultures.
Cometabolism made phenol and 2-chlorophenol simultaneous biodegradation. The time for complete removal of phenol and 2-chlorophenol was 8, 15, 23 and 25 days for initial phenol and 2-chlorophenol concentration of 17.2 mg/L and 14.3 mg/L,21.2 mg/L and 18.6 mg/L,25.3 mg/L and 23.4 mg/L as well as 30.4 mg/L and 29.1 mg/L, respectively. The experimental results of continuous-flow indicated that the removal efficiency reached 100% at steady-state from 96.5 to 100.5 h when the initial concentrations of phenol and 2-chlorophenol were 12.66 and 9.25 mg/L. The experimental results show that biofilm attached on ceramic particles process can effectively degrade phenol and 2-chlorophenol in wastewater.

並列關鍵字

Activated sludge ； biodegradation ； phenol ； 2-chlorophenol ； cometabolism

參考文獻

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