多孔鈦鈮鉬合金表面披覆氫氧基磷灰石增加細胞相容性之研究

Improving Cell Compatibility of Hydroxyapatite Coated Porous Ti-Nb-Mo Alloy

施旻宏(MIN-HUNG SHIH)

指導教授 ： 許學全

中臺科技大學/健康科學院/生物科技暨醫學工程研究所/碩士(2017年)

摘要

鈦合金由於具有良好的機械性質，耐腐蝕性和生物相容性而廣泛用於牙科和骨科植入物。 但是由於彈性模數與天然骨不匹配，會導致應力屏蔽效應，進而造成骨吸收和二次手術的風險。 人類皮質骨的抗壓強度在114〜195 MPa之間，骨植入物的孔隙率在30〜50 ％ 之間。 本研究製作多孔Ti-Nb-Mo 合金是採用空間保持器燒結法，並用電解沉積法塗覆氫氧基磷灰石（Ca10（PO4）6（OH）2;HA），以增加生物活性。 製作的多孔Ti-Nb-Mo 合金，其孔隙率為50 ％ （稱為TNM50），孔徑為101-250 μm，滿足骨向內生長的最佳尺寸範圍100-500 μm。 而抗壓強度為153 MPa，彈性模量為3.13 GPa。 HA 塗覆的多孔Ti-Nb-Mo 合金的孔隙率為50 ％ （稱為TNM50E5），抗壓強度為155 MPa，彈性模量為2.13 GPa。 TNM50E5 針狀鍍層的SEM / EDS 分析顯示Ca / P 比約1.67。 XRD 顯示這些塗層為HA 相組成，沒有任何其它磷酸鈣相。 細胞相容性方面，TNM50E5 具有比TNM50 更多的細胞附著性，WST-1 和ALP 分析結果顯示TNM50E5 比TNM50 較好的細胞活性，以及更多的細胞分化。
HA 鍍層之多孔Ti-Nb-Mo 合金(TNM50E5)具有合理的抗壓強度，良好的彈性模量和孔隙率，並表現優異的生物相容性，因此適合開發作為新植體材料。

關鍵字

多孔合金 ； 電解沉積 ； 氫氧基磷灰石 ； 細胞相容性

並列摘要

Titanium alloys are widely used in dental and orthopedic implants due to their good mechanical properties, corrosion resistance, and biocompatibility. However, there is a mismatch between the elastic modulus does not match the natural bone, resulting in a stress masking effect that can lead to bone absorption and the risk of secondary surgery. The compressive strength of human cortical bone is between 114 and 195 MPa, and the porosity of a bone graft is between 30 and 50%. In this study, the porous Ti-Nb-Mo alloy was fabricated using a space-holder sintering method and coated with hydroxyapatite (Ca10(PO4)6(OH)2; HA) using an electrolytic deposition process to increase bioactivity. The pore sizes of the alloys were in the range of 101–250 μm, which were within the optimal size range (100–500 μm) for bone ingrowth. The alloys also had a porosity of 50% (referred to as TNM50), a compressive strength of 153 MPa, and an elastic modulus of 3.13 GPa. Moreover, the HA-coated porous Ti-Nb-Mo alloys still had a porosity of 50% (referred to as TNM50E5), a compressive strength of 155 MPa, and an elastic modulus of 2.13 GPa. SEM/EDS analysis of the layers deposited on TNM50 shows a Ca/P ratio around 1.67 and a needle-like morphology. The XRD pattern shows that the layers have an HA phase composition, without any other calcium phosphate phases. SEM images show that TNM50E5 has more cell attachment than that of TNM50, while WST-1 results show greater cell activity. ALP analysis shows that TNM50E5 has more cell differentiation than that of TNM50.
The HA-coated porous Ti-Nb-Mo alloy (TNM50E5) has reasonable compressive strength, good elastic modulus and porosity, and excellent biocompatibility, and is therefore an excellent candidate for the development of new implant materials.

並列關鍵字

Porous alloy ； Electrolytic deposition ； Hydroxyapatite ； Cell compatibility

參考文獻

參考文獻

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