大黃蘆薈素抗病毒之功效與誘導第一型干擾素反應之研究

大黃蘆薈素（Aloe-emodin），已被證明在生物製劑上有許多功效，包括抗細菌、抗病毒、抗腫瘤之效果。本研究的目的在於使用試管內實驗來評估大黃蘆薈素抗病毒之功效。首先使用MTT方法分析藥物濃度百分之五十的毒殺效果 (50% Cytotoxicity Concentration)，以及使用細胞病變之MTT定量與病毒蝕斑試驗來分析大黃蘆薈素對日本腦炎病毒、腸病毒與流行性感冒病毒A型(H1N1) 之抑制百分之五十之病毒濃度(50% Inhibiting Concentration)。大黃蘆薈素對HL-CZ，TE671 及MDCK細胞之毒性低其CC50大於100 ug/mL，TE671、HLCZ細胞內抑制日本腦炎病毒之IC50分別為1.51 ug/mL、0.50 ug/mL，而抑制腸病毒71 型之IC50分別為0.14 ug/mL、0.52 ug/mL。在MDCK細胞內抑制A型流行性感冒病毒IC50為1.45 ug/mL。此外，大黃蘆薈素引發訊息傳遞路徑，藉由干擾素的報告基因證明能誘導細胞產生類似干擾素的傳遞效果。且有統計上的意義（ρ value <0.05），而利用即時定量PCR（Real-time PCR）顯示，在細胞處理大黃蘆薈素後，會造成PKR 與2',5'-OAS mRNA 表現量增加。ELISA的實驗中，當細胞分別處理大黃蘆薈素與病毒後均能誘導interferon α上升。另外，我們發現大黃蘆薈素可拮抗A型流行性感冒病毒NS1 之抑制interferon訊息傳遞路徑。本研究指出大黃蘆薈素促使 interferon type I生成ISRE及其下游因子表現與A型流行性感冒病毒NS1 蛋白所抑制NFκB下游interferon β 路徑不完全相同。由本實驗推論大黃蘆薈素之抗病毒機制可藉由誘發type I interferon訊息傳遞路徑達到抗病毒功效。

關鍵字

大黃蘆薈素

並列摘要

Aloe-emodin have been demonstrated to have various biological activities, including antibacterial, antiviral and anti-tumor effects. The goal of this study is to defermin the antiviral effects of aloe-emodin against Japanese encephalitis virus(JEV)，enterovirus 71(EV71) and influenza A (INF A) virus via in vitro assay. Aloe-emodin possessed low cytotoxicity to HL-CZ，TE671 and MDCK (50% cytotoxicity concentration CC50> 1000 ug/mL) cells. The 50% inhibiting concentration (IC50) of aloe emodin against JEV was 1.51 ug/mL and 0.5 ug/mL in TE671cell and HL-CZ cell,respectively. The IC50 against EV 71 was 0.14 ug/mL and 0.52 ug/mL in TE671 and HLCZ cell. In addition, the IC50 against INF A was 1.45ug/mL for aloe-emodin. Moreover, signaling pathway assay with cis-reproter vectors demonstrated induction of interferon (IFN)-triggered signal transduction pathways by aloe-emodin. Differences were considered significant (ρ<0.05). Real-time PCR analyses showed PKR and 2'5'-OAS mRNA levels from treated cells with aloe-emodin. ELISA analyses show that interferon α increases from treated cell with aloe emodin.

並列關鍵字

Aloe-emodin

參考文獻

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大黃蘆薈素抗病毒之功效與誘導第一型干擾素反應之研究

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