- 學位論文
利用RAPD 與ISSR 分子標誌評估台灣土肉 桂之遺傳歧異度
Evaluation of Genetic Diversity among Indigenous Cinnamon ( Cinnamomum osmophloeum KANEH.) by RAPD and ISSR Markerst
摘要
土肉桂(Cinnamomum osmophloeum KANEH.)又稱假肉桂，分類上屬 於樟科，樟屬，原產於台灣之樟科喬木，葉部富含肉桂精油，可應用 於醫藥、食品及化妝品。 本研究以分子層次之RAPD (random amplified polymorphic DNA) 與ISSR (inter simple sequence repeat)分子標誌進行17 個不同地區土 肉桂收集品系遺傳歧異度，更進一步探討土肉桂葉部肉桂醛含量，以 瞭解不同地理收集品系肉桂醛及遺傳分析間之關係。試驗結果顯示此 二種分析在土肉桂收集品系間均表現高度多型性。本研究用9 條 RAPD 引子共增殖103 個DNA 片段，其中80 個DNA 片段具多型性， 所產生的片段分子量大小介於3625 bp-250 bp 之間。而11 條ISSR 引 子共增殖124 個DNA 片段，其中105 個DNA 片段具多型性，所產 生的片段分子量大小介於2500 bp-250 bp 之間。以RAPD 與ISSR 所 產生多型性片段DNA 比率分別為77.67%與84.68%，其中ISSR 所產 生的多型性條帶比RAPD 多，且再現性較高。將9 條RAPD 引子及 11 條ISSR 引子所產生多型性DNA 片段，經Dice’s 公式計算其相似 性係數，並以UPGMA 建構相似度樹狀圖分析，結果顯示以RAPD 群集分析可分為7 群，遺傳相似度係數介於0.72~0.88 間，而ISSR 群集分析可分為5 群，遺傳相似度係數介於0.66~0.85 之間。
關鍵字
RAPD ISSR並列摘要
Indigenous cinnamon (Cinnamomum osmophloeum KANEH.) (Lauraceae) is a unique specie of camphor tree in Taiwan. The leaves of cinnamon are rich in essential oils which can be applied to medicine, food and cosmetics. The genetic diversity of seventeen accessions collected in Taiwan were evaluated by RAPD and ISSR markers.The results indicated that both methods revealed high degree of polymorphism and reproduction. A totally 103 amplified DNA fragments were generated by 9 selected RAPD primers and among them 80 fragments show polymorphic. The sizes of DNA fragments were between 3625bp and 250bp. A total 124 amplified DNA fragments, including 105 polymorphic fragments, were generated by 11 selected ISSR primers. The sizes of DNA fragments were between 2500 bp and 250 bp. The ratios of polymorphic DNA fragments produced by RAPD and ISSR were 77.67% and 84.68%, respectively. The similarity between accessions were estimated by using the Dice’s formula based on the total amplified and polymorphic DNA fragments from RAPD and ISSR. The genetic similarity was analysis by UPGMA (Unweighted Pair-Group mean Arithmetic). The results showed that UPGMA cluster analysis based on RAPD similarity matrixs could separate those varieties to seven distinct groups, genetic diversity were between 0.72 and 0.88. The ISSR similarity matrixs could separate those varieties to five distinct groups, genetic diversity were between 0.66 and 0.85
參考文獻
延伸閱讀
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